DNA Replication
DNA Technology
AHL Replication
AHL Enzymes
Pot Luck
100
production of exact copies of DNA with identical base sequences
What is DNA replication?
100
A technique used to amplify DNA fragments using cycles of temperatures
What is PCR?
100
The direction of action of DNA polymerases
What is 5' to 3'?
100
The function of DNA polymerase 3
What attaches to the RNA primer, and adds DNA nucleotides to a growing polynucleotide in the 5’ to 3’ direction.
100
A to T, G to C
What is complementary base pairing?
200
Each strand of the original DNA acts as a template for the newly forming strand
What is semi-conservative replication
200
The heat resistant enzyme used in PCR
What is Taq polymerase?
200
occurs on the leading strand
What is continuous replication?
200
This inserts an RNA primer onto the DNA strand
What is the function of DNA primase?
200
Relative amounts of pyrimidine and purine bases
What is Chargaff's rule?
300
Adenine binds to Thymine whilst Guanine binds to Cytosine
What is complementary base pairing?
300
This technique separates DNA fragments by size
What is gel electrophoresis?
300
DNA proofreading
What is the removal of mismatched nucleotides from the 3' terminal?
300
Removes the RNA primer, and inserts DNA nucleotides.
What is the function of DNA polymerase 1?
300
Organisation of DNA strands, using H bonds
What is the double helix?
400
This enzyme unwinds the DNA strands by breaking H bonds between nitrogenous bases
What is (the role of) helicase?
400
These are uses of PCR and gel electrophoresis
DNA profiling for paternity and forensic investigations...
400
Lengths of DNA made from the lagging strand
What is an Okazaki fragment?
400
Joins Okazaki fragments together.
What is the function of DNA ligase?
400
Naked
How is DNA found in prokaryotes?
500
This enzyme catalyses the formation of the new DNA strand through covalent bonding between nucleotides
What is DNA polymerase (III)?
500
Enzymes used to break DNA into fragments at specific base sequences. Used before Gel electrophoresis
What are restriction endonucleases?
500
The terminal end of the template DNA strand for the leading strand
Which is the 3' end?
500
once on the leading, repeatedly on the lagging strand
How often must DNA replication be initiated?
500
Eight (8)
How many histone proteins is DNA wrapped around in the nucleosome?