It's all About the Basement Membranes
Attack of the Cutaneous Appendages
Geno What?
Victoria's Stains
Structure is All You Need
100
Name at least one other name for Laminin 5
Epiligrin or Laminin 332
100
Correctly identify the mode of secretion for apocrine glands
Decapitation
100
What are the mutations in EBS?
Keratin 5/14.
100
Name at least one stain for melanocytes
S100, HMB45, Mart-1, Melan-A
100
Keratins are divided into two types. Name them.
Type 1 (Acidic) (K9-20). Type 2 (Basic) (K1-8).
200
The lamina densa is predominantly made of this material
Type 4 collagen
200
Identify the mode of secretion for sebaceous glands.
Holocrine
200
Name at least one gene involved in pachyonychia congenita
6A/16 in PC1. 6B/17 in PC2
200
Name at least one other cell type that is S100 except for melanocytes
Merkels, Eccrine, Langerhans, Nerve cells, Smooth and skeletal muscle. Rosai-Dorfman disease. Many others.
200
Name at least one molecule contained in keratohyalin granules
Loricrin, Pro-fillagrin, Keratins
300
Name at least two components of the hemidesmisome
Plectin. BP180 or BPAG2. BP230 or BPAG1. A6B4 integrin
300
Name at least two sites that contain apocrine glands
Axilla, Areola, Anogenital, Auditory canal, Eyelids (Glands of Molls)
300
Name at least two diseases wherein BPAG2 is an antigen
Bullous Pemphigoid (NC16A domain). Pemphigoid Gestationis (NC16A domain). Linear IGA (97 kd domain). Cicatricial Pemphigoid (distal C-terminal)
300
Name at least two stains for Merkel Cells
Bombesin, Chromogranin, low molecular weight cytokeratin 20, neuron-specific enolase, synaptophysin, S100
300
Name the two components of the stratum malpighian
Basal Layer & Spinous Layer. Take home point: Boards questions will sometimes use less common names.
400
Identify this molecule: One end attaches to Collagen 4 while the other end attaches to an antigen in cicatricial pemphigoid.
Nidogen
400
These glands secrete via merocrine secretion. Name the location where they empty their secretions.
Ecrrine glands. Directly on the skin surface.
400
This enzyme is involved in the assembly of the cornified envelope from precursors (loricrin, fillagrin, keratins). Name at least one disease in which this enzyme is mutated
The enzyme is transglutaminase. Mutations in transglutaminase 1 are implicated in Lamellar Ichthyosis and NBCIE. Acral peeling skin syndrome is due to mutated transgluatminase 5.
400
Carcinogenic transformation of these cells normally located in the basal layer is associated with infection by a polyoma virus. What stain, normally positive in these cells, becomes negative after carcinogenic transformation
Merkel cells. S100
400
The principal component of the sublamina densa is an antigen in two autoimmune diseases. Name both diseases.
Bullous SLE. Epidermolysis Bullosa Acquisita
500
How many kDa is the mutation in EBS with Muscular Dystrophy?
Plectin. 500 kDa.
500
These glands are principally under androgen control. They are located everywhere on the skin except over the ____?
Sebaceous Glands. Palms and Soles.
500
This ichthyosis is characterized by deficient steroid sulfatase, leading to accumulation of cholesterol-3-sulfate, prolonged labor in the mom, comma shaped corneal opacities and cryptorchidism in the affected child. What is the inheritance pattern?
X-linked Ichthyosis
500
Staining pattern: S100+, CD1A+, CD 207+. These cells have a characteristic structure seen on electron microscopy. What intra-cellular organelle constitutes the bulbous end of this characteristic structure?
Birbeck granules. Lysosome is the correct answer. FYI: The "racquet handle" of birbeck granules is derived from the cell membrane.
500
This keratin is expressed only on the palms and soles. What chromosome is it on?
17
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