DNA structure
Day 2
Day 3
100
The letter "A" is often used as an abbreviation of this nitrogenous base found in DNA
adenine
100
What are the components we add to PCR that first bind to the DNA in order to amplify the region of interest
Primers
100
What do we use to cut DNA at specific locations for gene editting?
Restriction enzyme
200
A purine contains this number of rings
2
200
In gel electrophoresis, heavier DNA molecules would be found closer to the _____ (cathode/anode)
cathode (-)
200
What is the name of the enzyme that patches up the DNA phosphate backbone to 'combine' new DNA into a plasmid?
DNA Ligase
300
In DNA, these two nitrogenous bases are pyrimidines
cytosine and thymine
300
What is the name of the enzyme that unwinds DNA during DNA replication?
Helicase
300
(T/F) We prefer to use a restriction enzyme that can create a blunt end during plasmid recombination
False (Prefer Sticky ends)
400
DNA wraps around this protein to form tightly packed nucleosomes
histones
400
What do you call the location of an allele on a chromosome?
Locus
500
DNA lacks an oxygen molecule at this carbon
C2
500
What does ddNTP stand for and what's the difference between a ddNTP and a dNTP?
Dideoxynucleotide Triphosphate
ddNTP lacks a OH at C3