Classical Genetics
A mother with genotype AaBb is crossed with a father with genotyope aabb. What is the probability that their kid has genotype aaBb?
9:3:3:1
Which stage of PCR has the highest temperature?
Denaturation
Name the 5 requirements for a population to be in Hardy Weinberg Equilibrium
Large population, random mating, no gene flow, no mutations, no natural selection
A sample of DNA has 34% cytosine. What portion of the DNA is made of uracil.
0%
What is Freddy’s favorite Hamilton song?
Take a Break
A mother with genotype AaBb is crossed with a father with genotyope aabb. What is the probability that their kid has genotype aaBb?
25%
Name one advantage of Illumina sequencing over Nanopore sequencing
Higher accuracy, higher throughput
What is the evolutionary mechanism that leads to the formation of analogous genes?
Convergent evolution
What is the net charge of a histone
Positive (+)
What is Emmanuel's star sign
Taurus
List all the blood types that are possible in the children of a type A mother and type B father.
A, B, AB, O
What is the main substance required for blue-white screening?
X-gal
What are the two types of homologous genes?
Orthologs and paralogs
Name 1 enzyme that has a 3' to 5' exonuclease and one enzyme that has a 5' to 3' exonuclease.
DNA polymerase I - 5' to 3'
DNA polymerase III - 3' to 5'
Which histone leads to the formation of the 30 nm fiber?
H1
Two genes are located on the same chromosome. Can these genes still follow Mendel’s law of independent assortment, and how?
Yes, if they are located far apart (more than 50 cM)
Name 2 differences between siRNA and miRNA
TBD
https://share.google/vEXkCIdexxC8B6rFu
Identify a monophyletic group, a paraphyletic group, and a polyphyletic group
TBD
What are the three types of gene sequences found in the bacterial origin of replication?
DnaA boxes, DUE (DNA-unwinding element), GATC methylation sites
What is the name of the DNA that holds nucleosomes together?
Linker DNA
What is an acentric fragment, and what is one mechanism by which one can be formed?
A chromosome without a centromere.
Ex. crossover of a chromosome with a paracentric inversion
You have 3 true-breeding strains of pea plants with white leaves, named Strain X, Strain Y, and Strain Z.
Crossing Strain X and Strain Y yields a plant with green leaves. Crossing Strain Y and Strain Z yields a plant with white leaves.
How many unique genes have been mutated across the three strains?
2
Describe the process by which an allopolyploid is formed.
Hybridization of gametes from different species followed by a doubling of chromosomes.
What type of histone methylation results in the formation of constitutive heterochromatin?
H3K4me3
What is emmanuel's favorite animal?
Henri