Introduction
Methods
Results
Random Heart Facts
RANDOM RANDOM
100
Cardiac muscle cell
What is a cardiomyocyte?
100
Detects the presence of protein according to size.
What does a Western blot do?
100
These cells can spontaneously differentiate into cardiomyocytes due to intrinsic factors.
What are BATDCs?
100
Shortness of breath, anxiety, lightheadedness, sweating, etc.
What are symptoms of a heart attack?
100
Uses fluorescently-tagged antibodies for imaging.
What is immunofluorescence staining?
200
Cardiac regeneration
What may serve as a potential treatment for myocardial ischemia/ infarction?
200
Beating, forming clusters, branching fibres, tight connections.
What are the four distinguishable features of cardiomyocytes?
200
Treatment with anti-Flt-1 and/or anti-Igf-1rα antibody decrease levels of phosphorylation of ____ in BATDC.
What is ERK1/2?
200
9 out of 10 hearts attacks are caused by this.
What is atherosclerosis?
200
These two molecules can be used to induce spontaneous cardiomyocyte differentiation in WATDCs
What are VEGF and IGF1?
300
Embryonic stem cells, induced pluripotent stem cells, bone marrow mesenchymal cells and adipose-derived stem cells
What cell types are known to contain cardiac progenitors?
300
This molecule was used as an internal control in Western blotting.
What is GAPDH?
300
Their addition resulted in an increase in expression of cardiac genes in WATDCs.
What did the addition of VEGF and IGF1 result in?
300
The obstruction of the blood supply to the heart or region of tissue of the heart, typically by a thrombus or embolus, causing local death of the tissue.
What is myocardial infarction?
300
Brown, beige and white.
What are the three types of adipose tissue in mammals?
400
Developmental signals and extracellular microenvironment.
What factors influence stem cell growth and differentiation?
400
This analysis shows that only one product was made.
What does the melting curve analysis do?
400
The phosphorylation levels of this protein did not change with the inhibition of the MEK/ERK pathway.
What is MEK1/2?
400
This disease is associated with the buildup of plaque in the arteries.
What is atherosclerosis?
400
BMPs, VEGF, IGF1, FGF.
Which molecules have the ability to promote cardiomyocyte differentiation in stem cells?
500
Adipose tissue is highly accessible and can be collected with ease.
Why did experimenters choose to use BATDCs and WATDCs to complete this study?
500
5% nonfat milk.
What substance was used for blocking in the Western blot?
500
mRNA levels of cardiac proteins are downregulated.
What does blocking VEGF or IGF-1 receptors do to BATDCs?
500
Blood flow to your heart is reduced, preventing it from receiving enough oxygen.
What is myocardial ischemia?
500
The differentiation of WATDCs was too slow when IGF1 and VEGF were added.
Why isn't cardiac regeneration a reasonable treatment right now?
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