Protocols
Ligase joining two ends of DNA
What is ligation?
Water that has no DNases or RNases
What is nuclease free water?
A circular piece of DNA commonly found in bacteria
What is a plasmid?
Amplifies DNA many times
What is PCR?
Something that helps a reaction go smoothly and may provide some of the necessary parts (e.g. ATP, nucleotides)
What is a buffer?
A plasmid with the "junk" removed
What is a backbone?
Restriction enzyme cuts DNA
What is a restriction digest?
It acts like "scissors" for a plasmid!
What is a restriction enzyme?
DNA moves through an agarose gel, forming bands based on size
What is gel electrophoresis?
It puts two single-stranded pieces of DNA together
What is oligo annealing?
Binds to specific parts of DNA to amplify them
What is a primer?
The process of putting a plasmid into cells
What is transformation?
It removes phosphate groups from the ends of DNA
What is a dephosphorylation reaction?
Originally derived from shrimp in the Antarctic
What is Antarctic phosphatase?
Ends of DNA that have overhangs that are complementary to each other
What are sticky ends?