Passages
What should you do if an EM plate is faulty?
1. Replace
2. Contact/Gloves plate can ONLY be replaced if the Inside operator is still in the hood
3. Contact Management as it may require a BF112
What do you do if the recipe gets stuck on a prompt?
Put it on hold and restart, answer prompt again
Call Automation
What do you with the remaining 50L after the 200 Inoc?
Save till you get 200 Day0 sample result
What should you do before you disconnect an empty media tote?
Clamp line downstream of the media to maintain sterility in case of a failed disconnection
What do you do as the bag inflates?
Manipulate the bag to get rid of creases and folds
Install stainless-steel plate to ensure bag is positioned correctly
Manipulate bag to ensure zip-ties on ports are not pinched between the bag and the support plate
Engage agitator once 500L of air is added
Remove hoist once 2000L of air is added
What do you do if you need to come out of the BSC after opening settle plates
Contact management as it may require a BF112
Get new plates and document everything in the MBR
What do you do if the bag becomes inflated?
Remove CBCU tubing to deflate
What do you do if the probe port connection is compromised?
Clamp the line and contact management
What do you do before you add a new media tote?
Check status then GI in SAP and record in MBR
What do you do if the flush sample bag is full?
Use the bag closest to the flush bag and label "Waste"
What do you do if you need to sneeze while in the BSC?
Turn your head away from the hood to sneeze
Rub the roof of your mouth with your tongue
What do you do if a recipe progresses to a step too early?
Contact a Sr colleague as recipe may need to be put on hold
Call automation
What do you do if an exhaust filter fails?
Clamp line and use backup exhaust filter
Order an additional filter in SAP
When installing Perfusion skid, what should you keep in mind about the Permeate flowmeter?
Always fit the skid to the flowmeter and never force it in, as it easily breaks
What do you if a luer lock comes off the sample line?
Clamp line closed
Use the other line
Weld on a different line
Use sample bags even before Tfxn
What do you do if the sample VCD is out of range?
Check SOP if it instructs to resample
Contact Management as it may require a BF112
Place a TO in SAP
Fill out emergency TO form in Popular Links
What do you do if a heating jacket fails?
Swap Jackets, there should be an additional one as recipe only runs 2
Place a WO
What needs to be done before you connect lines to the drain?
Make sure Permeate line is routed through the pump in the right direction, line can not be taken out of the pump after connection made to the drain
What do you do before selecting a pump in the equipment selection screen?
Go to pump screens and ensure it's connect and clear alarms
What do you do if a CO2 alarm goes off?
Stop everything and leave room immediately
What do you do if a pump is not showing in the selection screen?
Check to see if it's in the connected state on the pump screen
What do you do if condensation builds in an exhaust filter?
Open vent on the bottom of the filter and allow condensation to drain it
What do you look for during Perfusion flush?
Filters are prime and media is flowing from feed to retentate lines
Check for leaks or moisture
Who do you contact if a batch is not released in SAP?
Tiffany Rorer