Gel Electrophoresis
What is the area called where DNA is loaded into the gel?
The wells
What is the overall charge of DNA?
Negative
Say one place where DNA could be found at the crime scene.
Answers will vary
When is DNA cut, before it enters the gel or after it enters the gel?
Before
Who is the best teacher at MASH?
Mr. Ritchey
Where would you expect to find the shortest strands of DNA?
The furthest point from the start
How is DNA measured?
In base pairs
What is the first step of DNA analysis?
DNA extraction
What is the important step you must do when creating a gel for gel electrophoresis just before the gel is allowed to solidify?
Put the comb in to create the wells
STR analysis can always establish differences between identical twins or closely related individuals. True or False?
False
Where would you expect to find the longest strands of DNA?
Closest to the beginning
Where can DNA be found?
In the nucleus of the cells of living objects
What is the purpose of the extraction buffer?
It lyses the cells to expose the DNA
Name one common flaw that occurs when using gel electrophoresis?
Wells blow out, DNA runs off gel, etc.
What is the purpose of a ladder in a gel?
It is DNA of known lengths to compare to to create estimates for lengths of unknown DNA
How does gel electrophoresis separate DNA?
It uses charges. The negative DNA is attracted to the positive charge
Is DNA relatively stable or will it break down over time?
It is relatively stable
What is the process used to create extra copies of DNA?
Polymerase Chain Reaction
Once the gel has finished running, what is the final step to analyzing the DNA?
Compare bands to establish a match
The only DNA that ever matters in Forensics is human DNA. True or False?
False
What would happen if DNA was loaded into the positive end of the gel?
The DNA would run backwards off the gel
Individuals have different combinations of lengths of VNTRs and STRs which can be used to identify them. True or False?
True
What is responsible for cutting DNA at specific points?
Restriction Enzymes
List the three steps of DNA analysis in the correct order.
1. DNA extraction
2. PCR
3. Gel Electrophoresis
What is another term for DNA profiling?
DNA Fingerprinting