Southern Blotting
DNA Structure
History
Definition
Random
100
PCR
Polymerase Chain Reaction
100
Adenine - Thymine (A-T) Cytosin - Guanine (C-G)
Nitrogen bases
100
The year DNA fingerprinting was created.
1984
100
The use of a DNA probe for the identification of an individual, as for the matching of genes from a forensic sample with those of a criminal suspect.
DNA Fingerprinting
100
Name the two disorders mentioned that can be diagnosed with a DNA fingerprint.
Cystic fibrous and Sickle cell anemia
200
Example of samples needed for DNA analysis
Tissue; blood, hair, skin, etc.
200
DNA
Deoxyribonucleic Acid
200
The inventor of DNA fingerprinting.
Professor Sir Alec John Jeffreys
200
The process by which DNA fragments are transferred from agarose gel to nylon sheets.
Blotting
200
The DNA fingerprinting method is now considered better than what?
Dental records and Blood typing
300
Product used for electrophoresis which is made from seaweed extract
Agarose gel
300
Used to retrieve the DNA from the nucleus of a cell.
Micropipette
300
Exonerated in two rape-murder cases because DNA didn't match. First to be proven innocent based on DNA.
Richard Buckland
300
An enzyme that attacks a specific sequence of nitrogenous bases on a DNA molecule to create fragments of DNA with unpaired bases.
Restriction Enzyme
300
Professor Sir Alec John Jeffreys was knighted by Queen ELizabeth II for what?
Services to Science and Technology
400
DNA is transferred from agarose gel to _______.
A nylon sheet
400
The carrier of genetic information.
DNA
400
The year the first paternity case was solved in.
1985
400
RFLP's
Restriction Fragments Length Polymorphisms
400
How many steps are involved in the process of Southern Blotting?
Six steps
500
Radioactive particles that join to DNA fragments. Are used to produce a banding pattern known as a DNA fingerprint.
Probes
500
Encrypted sets of numbers that reflect a person's DNA makeup.
DNA profiles.
500
________ in _______ was the first person ever convicted of a crime on the basis of DNA evidence.
Robert Melias, 1987
500
The process by which DNA fragments are sorted using an electric current.
Electrophoresis
500
What is placed over the nylon sheet? What happens to it?
A photographic film is placed over the nylon sheet and formed darks bands when the film is developed.
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