Extraction
This extraction method involves the use of a chemical solvent (phenol/chloroform) to separate double-stranded DNA from cellular protein and debris.
What is organic extraction?
Only this type of scalpel may be used to cut evidence swabs.
What is Sterile?
20-50 microliters of this reagent is added to the center of the silica membrane of the QIAamp Minelute column.
What is Tris-EDTA (TE)?
This must be checked/verified before use of any chemical in the laboratory used in case work.
What is the Reagent Log?
The Robotic Extraction instrument, QIACube (Connect), begins extraction at the addition of this reagent.
What is Buffer AL?
This extraction method uses a silica membrane or beads to reversibly bind DNA in a sample.
What is solid phase extraction?
If in doubt, you should do this before touching an item of evidence.
What is change gloves?
You must perform at least this many elutions.
What is two?
This log contains profiles that have been generated during casework but do not qualify for a CODIS, LDIS, NDIS, etc. classification. This information is stored as a contamination detection tool.
What is the Profiles Generated Index (PGI)?
After adding these two reagents at the Lysing Step of extraction, you will incubate the samples (1-18 Hours for Reference Samples, and 3-18 Hours for Evidence), and then centrifuge the samples at 10,000xg for one minute.
What is ATL and Pro-K?
The QIAamp Micro extraction method is only used for this type of sample.
What is/are Reference Sample(s)?
This/these sample(s) are initiated at the lysis phase of extraction. It contains all reagents, but no added DNA.
During elution, what speed should you centrifuge your sample?
What is full speed?
These solutions are used to clean any utensils used to cut or manipulate the swabs or other types of evidence.
What is 10% bleach and 70% ethanol?
This reagent is used in the "Washing" step of extraction. It contains a lower salt level than previously added reagents. It is followed by one to two more reagents, depending on the extraction method.
What is AW1?
This extraction method involves using a chelating-resin suspension to pull out Magnesium from the solution, effectively inactivating nucleases and protecting the DNA.
What is the Chelex method?
This is where contamination occurrences are tracked.
What is the Contamination Log?
After adding the low-salt, pH neutral reagent to the column, the sample must incubate at this temperature.
What is room temperature?
Final DNA extractions should be labeled with this information.
What is case number, exhibit number, and analyst's initials?
This reagent is used in the "Wash" step of the Investigator Manual Extraction method, but is omitted from the Micro Manual extraction method.
What is ethanol?
During the "wash" step of any manual extraction performed at ATF, two wash reagents are used. Which one of the wash reagents contains the lower level of salt?
What is AW1?
These cleaning agents are used to decontaminate a work bench or hood.
What is 10% bleach (BleachRite) and 70% Ethanol?
During incubation, the sample must sit for this long.
What is ten minutes?
Before using a reagent, you must verify these three things.
What is Volume, Expiration Date, and Reagent Log?
The volume of this reagent should be half of ATL volume. It is added at the "Binding" step.
What is ethanol?