Cell Growth
In Vitro(PCR)
In Vivo(in cells)
Leading or Lagging
Easy Question
"ase"y Answers
(all answers will contain "ase")
"ase"y Answers
(all answers will contain "ase")
200
When the net population of bacteria is increasing.
What is log or exponential phase?
200
Denaturation, annealing, and extension (DNA synthesis).
What is the PCR amplification cycle?
200
One strand being the parent strand and one being the daughter strand.
What is semiconservative DNA replication?
200
This strand consists of continuous DNA replication as it approaches the replication fork.
What is the leading strand?
200
Unwinds the DNA helix by breaking the hydrogen bonds between strands.
What is Helicase?
400
When there is more waste in the environment than nutrients resulting in a net decrease in cells.
What is the death phase?
400
The "artificial" substitution for helicase.
What is denaturation, or breaking the bonds holding the DNA strands together using heat?
400
The direction of DNA syntheses.
What is 5' to 3'
400
On both strands, this initiates synthesis.
What is the RNA Primer?
400
Synthesizes DNA on both the leading and lagging strand in the 5' to 3' direction.
What is DNA polymerase III?
600
When cell growth is net zero.
What is both lag and stationary phase?
600
A Current common use for PCR testing.
What is a Covid-19 PCR test?
600
Circular DNA replications occurs in ______, whereas linear DNA replication occurs in _______.
Prokaryotes (bacteria cells), Eukaryotes
600
The small fragments that are created from discontinuous replication, which are later bonded together.
What are Okazaki Fragments?
600
Synthesizes the RNA primers for both the leading and lagging strands.
What is primase?
800
When Binary fission is occurring.
What are both exponential (log) and stationary phases?
800
The process of cooling the previously heated DNA strands and binding them with primers.
What is annealing?
800
Each replication bubble has this number of origins of replication.
What is one?
800
Where the leading strand will be synthesized.
What is the bottom strand?
800
Responsible for replacing the RNA primers with DNA nucleotides at the end of replication.
What is DNA Polymerase I?
1000
A controlled environment with set amounts of nutrients, and a set amount of time.
What is batch culture?
1000
The thermostable DNA polymerase used for in vitro replication.
What is taq polymerase?
1000
In the eukaryotic cell cycle DNA replication only occurs in this phase.
What is the S phase?
1000
The last step of DNA replication for the lagging strand, occurs after the RNA primers are removed.
What is catalyzing phosphodiester bonds to join DNA fragments, this is completed by ligase.
1000
Helps the DNA unwinding process by relaxing the supercoiled DNA.
What is topoisomerase?