Enzymes
What does DNA Ligase do?
Connects the Okazaki fragments in the lagging strand
What happens after the Helicase breaks the hydrogen bonds?
Single Stranded Proteins hold the two strands separately
What is RNA primer
An RNA nucleotide used to anchor the DNA strand to parental strand and used to start the DNA replication
What does DNA polymerase III do?
Adds nucleotides starting from the RNA primer
What happens after the replication bubble is formed at the Origin of Replication?
Topoisomerase binds to the DNA strand
The separation of the strands after the hydrogen bonds are broken by helicase
What does RNA Primase do?
Adds the RNA primer at the start of replication
What happens after Single Stranded Proteins separate the strands?
RNA primase attaches an RNA primer to the two strands
What is the Origin of Replication
Area in DNA where replication will begin
What does Topoisomerase do?
Controls the rate of winding and unwinding of the strand
After Exonuclease what happens?
DNA Polymerase I places DNA nucleotides where the RNA primer was originally
What is the Leading Strand
3' --> 5' of the parent strand or 5'-3' of daughter strand. One continuous strand of DNA is formed.
What does Exonuclease do? (Two things)
Removes the RNA primers AND any incorrect base pairs
For the lagging strand, what happens after DNA polymerase III is done adding nucleotides?
Exonuclease removes the RNA primers and any incorrect DNA base pairs
The sliding clamp pushes or moves DNA polymerase III along the DNA template strand