Enzymes
DNA Polymerase
Which base pairs with A?
T
image on slide 30
What enzyme is shown?
primase
Where DNA replication starts
origin
Order the enzymes based on the order of steps in DNA replication
Primase, helicase, ligase, DNA polymerase
helicase, primase, DNA polymerase, ligase
Which enzyme functions before DNA polymerase
a. primase
b. ligase
a. primase
Which base pairs with C?
G
image 4 from worksheet sequence with okazaki fragments unlabeled
label the diagram
okazaki fragments
what is the shape of DNA
double helix
image of different models of replication
which model shows semi conservative replication?
Which enzyme acts like "glue"
Ligase
5' ATGCGTC 3'
image on slide 14
label the parent and daughter strands
parent = dark blue, daughter = light blue
Image in slide 34
identify the replication fork
right where the DNA splits
Why does DNA need to replicate?
So each new cell has DNA when the cell divides
What is the function of Helicase?
Unzips DNA
What are the three components of a DNA nucleotide?
Phosphate, sugar and nitrogenous base
image of DNA polymerase adding bases without a primer
image of DNA polymerase adding bases 3' to 5'
what is wrong with each image?
No primer
3' to 5' instead of 5' to 3'
what is the job of single strand binding protein?
Keeps single stranded DNA in place
Which strand do okazaki fragments occur on?
lagging
Which way does DNA polymerase add bases on the lagging strand?
a. 5' --> 3'
b. 3' ---> 5'
a. 5' --> 3'
What one word describes that one DNA strand is 5' to 3' and the other is 3' to 5'
antiparallel
first panel of image in slide 41
what happened?
mutation
Define semi conservative
What are two things that could go wrong in DNA replication?
Possible answers (+more)
skip a base, add a base, add the wrong base, DNA gets tangled, enzyme falls off of DNA