Proposed Models of DNA Replication
In this model of replication, the original DNA strands reform the original double helix, while the two new DNA strands form a new double helix.
Conservative replication
This enzyme unwinds the DNA double helix at the replication fork.
Helicase
This enzyme adds a short strand of RNA nucleotides known as a primer.
Primase
This strand is synthesized continuously.
What is the leading strand?
How is DNA cut up to create a DNA fingerprint?
Restriction enzymes
Which is the Earth's largest continent?
Asia
This enzyme adds DNA nucleotides to the 3' end of an elongating strand.
DNA polymerase III
What does GDP stand for in economics?
Gross domestic product
This strand is composed of Okazaki fragments.
What is the lagging strand?
What tool can separate pieces of DNA by size?
Gel electrophoresis
In this model of replication, one old DNA strand combines with one new DNA.
Semiconservative replication
What poisonous spider can be identified by a small red hourglass-shaped marking on the underside of its body?
Black widow
This enzyme relieves supercoiling ahead of the replication fork.
Gyrase
Describe the directionality of DNA replication
5' to 3'
What was the period of American history when alcohol was banned?
Prohibition
Who were the scientists that determined the correct model of DNA replication?
Meselson and Stahl
This enzyme proofreads the new strands and fixes mistakes
DNA polymerase III
This enzyme removes the RNA nucleotides of the primer and replaces with DNA nucleotides.
DNA polymerase I
Which pair of German brothers collected such stories as "Hansel & Gretel"?
The Brothers Grimm
Give an example of when PCR would be a valuable tool.
Covid testing, DNA fingerprinting, testing for the presence of species
In this model of replication, the original DNA strands break into pieces and the new DNA reassembles as a mixture of old and new DNA.
Dispersive replication
This enzyme creates one continuous strand of nucleotides at the end of DNA replication
Ligase
These proteins keep the strands separate during replication
SSBP
This strand requires a primer.
What is the leading strand AND lagging strand?
Why are enzymes like taq polymerase used in PCR?
Can withstand high heat