Enzymes
Replication Facts
PCR
Electrophoresis
Toss Up
100
This enzyme adds new DNA bases to the template strand of DNA
DNA Polymerase III
100
What is the name for the location where DNA replication occurs?
The Replication Fork
100
What is the purpose of PCR?
To make millions of copies of DNA in a short period of time
100
State two applications of gel electrophoresis
Paternity testing and Forensics
100
Because each new strand of DNA created in replication contains half of the template strand, DNA replication is said to be...
Semi-conservative
200
This enzyme "unzips" DNA
Helicase
200
DNA Polymerase III moves continuously down this strand of DNA
The Leading Strand
200
What is used to separate the two strands of DNA?
Heat (breaks hydrogen bonds)
200
Which fragments travel the furthest across the gel plate?
The fragments with the fewest base pairs
200
Who was determined to be baby Harshithaa's father?
Mr Sravya Saranu
300
This enzyme prevents supercoiling
DNA Gyrase
300
Most of the dust found in your house is actually this:
Human skin cells
300
What is used to add new nucleotide to the separated strands of DNA?
TAQ Polymerase
300
State the charge on DNA and list which part of each nucleotide carries that charge
Negative, phosphate group
300
List two applications of PCR
Medical testing, DNA profiling, genetic disorder testing, paternity testing, etc.
400
This enzyme replaces RNA bases with DNA bases during DNA replication
DNA Polymerase I
400
What is the name of the portions of DNA found on the lagging strand of DNA?
Okazaki fragments
400
Where was TAQ Polymerase discovered?
Hot springs in Yellowstone
400
Who is the father?
Dad #3
400
Which strand of DNA requires ligase more frequently?
The lagging strand
500
This enzyme adds RNA bases to the template strand of DNA
Primase
500
Why can't DNA Polymerase III move continuously down the lagging strand?
DNA Polymerase III can only add nucleotides in a 5' to 3' direction
500
List or draw the full process of PCR:
500
Who is the criminal?
Suspect #2
500
Name Farmington Public School's superintendent
