What does DNA stand for?
Deoxyribonucleic acid
What are the 4 nitrogen bases in DNA?
Spell them out
Adenine (A)
Thymine (T)
Guanine (G)
Cytosine (C)
What shape is DNA?
Double Helix
Which strand is built in fragments during replication?
Lagging strand
Enzyme that unzips the DNA molecule.
What bonds does it break in the DNA?
Helicase
Hydrogen bonds
What sugar is in DNA?
Deoxyribose
What does A pair with?
Thymine (T)
What does C pair with?
Guanine (G)
Location where helicase breaks hydrogen bonds.
Replication Fork
Which strand is built continuously?
Leading strand
What does semi-conservative replication mean?
Each new DNA molecule has one old strand and one new strand
What types of bonds hold the nitrogen base pairs together.
How many for A-T
How many go G-C
Hydrogen
A-T = 2
G-C = 3
If a strand has 30% A, how much T is there?
What % will G and C be as well?
A and T = 30%
G and C = 20%
What strand is made in the same directions as helicase
What stands is make in the opposite direction as helicase
Leading strand
Lagging strand
What must initiate DNA replication?
RNA Primer
What individuals are most well known for discovering the shape of DNA
Rosalind Franklin, James Watson, and Francis Crick,
What rule explains base pairing?
What does it say?
Chargaff's rules
The amount of adenine (A) in the DNA of an organism = the amount of thymine (T)
Similarly, whatever the amount of guanine (G), the amount of cytosine (C) is the same
What makes up the backbone of DNA?
What bonds hold the nucleotides together?
Sugar / Phosphate back bone
Covalent bonds hold it together
Enzyme that binds free floating nucleotides to the growing DNA chain.
DNA Polymerase
enzyme that joins DNA fragments on the lagging strand
DNA Ligase
Put these in order: Primase, Helicase, Ligase, DNA Polymerase
Helicase → Primase → DNA Polymerase → Ligase
Draw and label the 3 parts of a nucleotide
What 2 parts of a nucleotide remain the same
What part can change?
The sugar and phosphate remain the name
The nitrogen base can change and be a A, T, C, or G
What does the term antiparallel mean
Which ends of the DNA go together?
The two stands that make up a DNA molecule run in opposite directions
One strand runs from 5' to 3', while the other runs from 3' to 5'
5' end of one strand aligns with the 3' end of the other.
In what direction is DNA built?
5' to 3' direction
What is the name of the sections of DNA being made only on the lagging stand
Okazaki fragments