Gene Cloning
cDNA & Libraries
PCR
Blotting
Biotechnology
100
A plasmid is a natural example of this used in gene cloning.
What is a vector
100
This enzyme creates DNA from RNA.
What is reverse transcriptase
100
Developer of PCR in 1985
Who is Kary Mullis
100
This type of blotting is used with RNA.
What is Northern Blotting
100
an organism with recombinant DNA in its genome
What is transgenic
200
These enzymes produce sticky ends
What are restriction enzymes
200
cDNA is advantageous because it lacks these
What are introns
200
This consists of a sequence of 15-25 bases and is crucial in the annealing step
What is a primer
200
Radiolabeled cloned DNA is used in this blotting procedure.
What is Southern blotting
200
this occurs when a defective gene replaces a good copy (often used in research)
What is gene knockout
300
An antibiotic resistance gene in your vector is an example of this.
What is a selectable marker.
300
This is used to determine the location of restriction sites on a vector
What is restriction mapping
300
This step of PCR requires the use of Taq polymerase to build the new strand.
What is extension
300
Western blotting can be used to determine if a protein is used in a particular cell or this.
What is a specific stage in development
300
This bacteria produces crown galls and is used to genetically modify plants.
What is Agrobacterium tumefaciens
400
This gene encodes beta-galactosidase, which is used to identify transformed bacterial colonies.
What is lacZ
400
This contains hybrid vectors with cDNA inserts
What is a cDNA library
400
This type of PCR starts with RNA to generate cDNA
What is reverse transcriptase PCR (RT-PCR)
400
Proteins must be separated using this type of electrophoresis.
What is SDS-PAGE
400
If the T-DNA of the Ti plasmid is not inserted into the plant's DNA, this will be the result on the selective media (plant life).
What is the plant will not have the gene of interest, nor will it grow on selective media.
500
If your colonies are white, it indicates this.
What is successful uptake of the gene of interest (successful transformation).
500
A restriction map is generated from cutting plasmids with restriction enzymes and this other method.
What is DNA sequencing
500
In real-time PCR, this molecule prevents the reporter from fluorescing.
What is the quencher
500
A northern blot which yields three different molecular weights of the same mRNA in three different tissues would indicate this about that mRNA.
What is alternative splicing
500
The genes of Bt corn allow for the expression of genes related to this.
What is chemical production to kill insects.
M
e
n
u