RNA/DNA origami
These changes make DNA more chemically stable than RNA, and possible to repair by the DNA repair machinery.
What is the effect of RNA having an extra hydroxyl group in the 2’ position and replacing thymine with uracil (methyl group is lost.)
An assembly technique to make polymer capsules.
What is the layer-by-layer (LbL) technique?
The most common building block of liposomes
What are phospholipids?
Common structural component of the cell membranes.
What is cholesterol?
The design and construction of nanostructures using nucleic acids (DNA or RNA) as building block.
What is DNA or RNA nanotechnology?
Chemical group that can make polymer capsules swell when changing the pH from 4 to 7.
What are COOH groups?
First type of liposomes that form when using the rehydration method
What are LMVs (Multilamellar vesicles)?
Temperature required to induce a change in the lipid physical state.
What is the phase transition temperature?
DNA origami requires a long strand (scaffold) that is shaped by different smaller staple strands. It requires thermal annealing/ folding. RNA origami commonly consists of one single-stranded RNA co-transcriptionally folded (gradually folded during transcription).
What are the differences between DNA origami and RNA origami?
The type of interactions that allows to load DNA into polymer capsules.
What are electrostatic interactions?
Polymer often used to make liposomes stealth.
What is PEG?
Thickness of a lipid bilayer in nm.
What is 4 nm?
A reaction between a duplex RNA or DNA, containing a toehold, that forms a thermodynamically more stable duplex with another (invader) single-stranded strand.
What is a strand displacement reaction?
The two energies that dictate if a liposome can form.
What is edge energy and bending energy?
Lipids that show high lateral mobility in a bilayer.
What are unsaturated lipids?
A short single-stranded oligonucleotide (DNA or RNA), selected by a method termed SELEX, that binds with high affinity and specificity to cognate targets.
What is an aptamer?
Thermodynamically speaking the two reasons for protein resistance on a PEG-surface.
What is entropic repulsion and osmotic repulsion?
‘Chemical backbone’ in a phospholipid.
What is three-carbon glycerol?