Gram-Positive Cocci in Clusters
Gram-Positive in Cocci
Gram-Positive Bacilli
Media and Plates
Biochem Logic
100

This enzyme differentiates Staphylococcus from Streptococcus. 

What is catalase?

100

The hemolysis pattern is the most commonly associated with Streptococcus pyogenes.

What is beta-hemolysis?

100

This GPR is spore-forming and commonly found in soil.

What is Bacillus species?

100

This medium is selective and differential for Staphylococci species.

What is mannitol salt agar (MSA)?

100

This enzyme forms a fibrin clot in blood plasma.

What is coagulase?

200

A catalase-positive, coagulase-positive organism is most consistent with this species.

What is Staphylococcus aureus?

200

This test differentiates Enterococcus from non-enterococcal streptococci

What is bile esculin?

200

This organism demonstrates tumbling motility at room temperature. 

What is Listeria monocytogenes?

200

This medium enhances metachromic granules in Corynebacterium diphtheriae.

What is Loeffler medium?

200

This biochemical test differentiates catalase-negative streptococci that can survive in oxygen from strict anerobes.  

What is peroxidase?

300

A coagulase-negative, novobiocin-resistant organism is most consistent with this species.  

What is Staphylococcus saprophyticus?

300

This organism is PYR-positive and bacitracin-susceptible.

What is Streptococcus pyogenes?

300

This GPR is catalase-positive, non-spore-forming, and part of normal skin flora.

What is Corynebacterium species?

300

Black colonies with brown halos on this medium indicate tellurite reduction.

What is Tinsdale agar?

300

A positive result on this test explains survival in high-salt environments.

What is catalase?

400

This virulence factor allows S. aureus to evade opsonization.

What is Protein A?

400

This organism is alpha-hemolytic, optochin-sensitive, and bile soluble.

What is Streptococcus pneumoniae?

400

This organism forms spores and exhibits a “Medusa head” colony morphology.

What is Bacillus anthracis

400

This test demonstrates enhanced hemolysis when S. agalactiae is streaked near S. aureus.

What is the CAMP test?

400

This enzyme protects organisms from oxidative damage by converting superoxide radicals.

What is superoxide dismutase?

500

A blood culture from a patient with a prosthetic heart valve grows gram-positive cocci in clusters. The organism is catalase-positive, coagulase-negative, and novobiocin-susceptible. What is the most likely organism?

What is Staphylococcus epidermidis?

500

A wound culture grows beta-hemolytic gram-positive cocci in chains. The organism is PYR-negative and bacitracin-resistant.

What is Streptococcus agalactiae?

500

A pregnant patient presents with flu-like symptoms. Blood culture grows a gram-positive rod that is catalase-positive, beta-hemolytic, non-spore-forming, and demonstrates tumbling motility at room temperature. What is the most likely organisms?

What is Listeria monocytogenes?

500

A throat culture is submitted for suspected streptococcal infection. The lab technician uses a non-enriched medium and is unable to determine hemolysis patterns. What medium should have been used?

What is blood agar?

500

A bacterium retains crystal violet-iodine complex after alcohol decolorization. What biochemical property explains this?

Thick peptidoglycan layer with extensive cross-linking

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