Chemistry
protocols
limitation
technique
real world cases
100

What functional groups allow amido black to bind with 

proteins? 

Amido and sulfonic acid groups 

100

Name a commonly used fixative before applying Amido black ?

Methanol or acetic acid. 

100

Why might Amido black fail to detect blood on certain surfaces ?

Highly textured or absorbent surfaces may obscure results 

100

How is Amido black used in combination with finger print detection methods ?

used after Cynoacrylate fuming or ninhydrin for enhancing bloodstain 

100

What type of criminal investigation frequently use Amido black? 

Crime involving Blood 

200

What is the pH range where Amido black stain is most effective ?

pH 6.8 to 7.2 

200

Why is it important to fix bloodstains before applying Amido black ? 

To prevent smearing or loss of blood evidence during staining 

200

What is one disadvantage of using Amido black compared to luminol ?

Luminol is more sensitive and works on invisible stains with out background staining 

200

Name a surface where Amido black is particularly effective for latent bloodstains ?

Porous surfaces like paper or cloth

200

Amido black helped confirm bloody footprints in which type of scene ?

Scene involving tracking of someone injured or an assault took place 

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