Analytical Techniques
Components of Spectrophotometer
Carbohydrates
Carbohydrate Metabolism Regulation
Methods of glucose determination
100
The lamps most commonly used for ultraviolet work is _____________?
deuterium-discharge lamp
100
Reduces stray light and prevents scattered light from entering the monochromator.
What is Entrance slit
100
Stereoisomers, Number of sugar, functional groups, and chemical properties of carbohydrates.
Classification of carbohydrates
100
Decreases the entry of glucose into the cells & increasing glycolysis.
What is Growth hormone
100
Condensation with aromatic amines
What is Ortho-toluidine?
200
Used to measure concentration by detecting absorption of electromagnetic radiation by atoms.
Atomic Absorption Spectrophotometer
200
Separates white light into a continuous spectrum through refraction for visible light and UV range.
What is Quartz Prism?
200
Breakdown of glycogen to glucose for use as energy
What is Glycogenolysis?
200
source of Thyroxine.
What is Thyroid gland?
200
Stabilizer: citrate or tartrate
What is Benedict’s and Fehling’s tests
300
Is the migration of charged solutes or particles in an electrical field.
What is electrophoresis?
300
Converts transmitted radiant energy into an equivalent amount of electrical energy
What is photodetector?
300
Glucose is broken down into two three-carbon molecules of pyruvic acid.
Embden Meyerhof Pathway
300
Increased in glycogenisis, glycolysis entry of glucose into muscle & adipose cell.
Mechanism of Insulin.
300
Uses BaS0 to remove non-glucose reducing substance
What is Nelson Somogyi
400
Is used to separate mixtures of compounds that are volatile or can be made volatile.
What is Gas Chromatography?
400
Most common source for the visible and near IR region.
What is Tungsten Iodide Lamp?
400
Ketonemia
What refers to the accumulation of ketones in blood.
400
Mechanism and source of Epinephrine.
What inhibits insulin secretion increasing glycogenolysis; Adrenal Medula
400
Mutarotase, Glucose Oxidase, Peroxidase
Enzymes that is involve in Glucose Oxidase
500
Is used for the analysis of biomolecules, such as peptides and proteins.
What is Matrix-assisted laser desorption ionization time of flight (MALDI-TOF)?
500
also known as cuvette or analytical cell holds the solution to be measured.
What is sample cell?
500
Glycosylated Hemoglobin term used to describe the formation of a ___________________ produced when glucose reacts with the amino group of hemoglobin.
hemoglobin compound
500
it is in the adenohypophysis.
What is growth hormone
500
Hagedorn jensen
What is Ferric reduction method?
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