Past LRQs
Week 1-6 Class Slides/Guided Inquiry
Weeks 6-13 Class Slides/Guided Inquiry
Genetic Engineering/DNA Party
LRQs from the first few weeks of the semester
100
A group composed of all species descended from a common ancestor
What is a clade?
100
The three parameters for evolution by natural selection
What is variation, heritable traits, and differential reproductive success?
100
This forms between non-sister chromatids at the point of crossing over
What is a chiasma?
100
You should look for this to find a viable restriction enzyme cutting site
What is a palindromic sequence?
100
These act by killing microbes or by preventing microbes from reproducing
What are antibiotics?
200
These bind to DNA by binding to the 3D shape of both strands in the DNA double helix
What are transcription factors?
200
The frequency of these will not change over generations for a population in Hardy-Weinberg equilibrium
What are alleles?
200
This is represented by a microarray column, and this is represented by a microarray row. (Two part answer!)
How is an experiment's data represented in a microarray? How are genes' data represented in a microarray?
200
Restriction digests, sequencing, and blue/white screening will all aid in selection of this
How do we select for positive transformants?
200
The cytoskeletal component with the largest diameter
What is a microtubule?
300
Translation of an mRNA starts here
What is a start codon?
300
What direction must DNA polymerase move along the template strand in order to replace RNA primers with DNA?
What is 3' to 5'? *Remember that this is so that the newly synthesized DNA strand is formed in the 5' to 3' direction*
300
Why microarrays have thousands of spots on them with single stranded DNAs
What is base pairing with cDNAs from every gene in an organism?
300
The process is as follows: 1. Isolate mRNAs 2. Synthesize cDNA 3. Make cDNA double stranded 4. Make recombinant plasmid 5. Transformation
How do you create a cDNA library?
300
They both synthesize proteins using ribosomes, can communicate with other cells, and undergo metabolic chemical reactions
What are eukaryotic and prokaryotic cells?
400
This contains a common ancestor and some but not all of the descendants of that ancestor
What is a paraphyletic taxon?
400
These non-organelles require an RNA template to complete their task. They are located in several places throughout the cell, including mitochondria.
What are ribosomes?
400
These, not genes, determine the characteristics of an organism
What are the RNA and proteins produced by a gene?
400
These three enzymes are necessary to make a recombinant plasmid. (The third one is tough to figure out, so 50+ points if you get all three)
What are restriction enzymes, DNA ligase, and phosphatase? (Phosphatase helps prevent vectors from ligating back onto themselves without an insert by removing phosphates on the 5' ends of DNA)
400
These line up individually in the middle of the spindle during metaphase in mitosis
What are homologous chromosomes?
500
Replication of these two things cause many identical copies of genes to be cloned in bacteria
What are plasmid and bacterial cell replication?
500
These twisted, ropelike filaments do not aid in cell movement.
What are intermediate filaments?
500
An example of this is: fetal hemoglobin has a higher affinity for oxygen than adult hemoglobin because this facilitates the transfer of oxygen from the mother to the fetus
What is gene regulation?
500
You would use this enzyme for genetic engineering if you wanted to make 'intronless' copies of your gene
What is reverse transcriptase?
500
According the Surface Metabolism model of chemical evolution, these likely catalyzed the formation of early organic molecules
What are the minerals which make up the deep sea vents?
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