This gene allows for selection of cells that contain the topo plasmid.
What is bla gene (beta-lactamase)
100
The activity of enzymes can be changed by binding to this location.
What is an allosteric site?
100
This property of Taq polymerase makes it ideal to use in PCR reactions.
What is heat stable
100
An agarose gel separates DNA based on this characteristic.
What is size (length)
100
The winner in a fight between hydra and daphnia.
What is hydra
200
The color of colonies that contain the topo plasmid without the PCR insert.
What is blue?
200
These enzymes are named after certain bacterial strains that had the ability to resist bacteriophage infection.
What is a restriction enzyme?
200
The step in PCR where hydrogen bonds between DNA strands are broken.
What is denaturation?
200
Agarose is a type of this class of biomolecule.
What is polysaccharide?
200
This gene is found in all species of microorganisms and can be used to classify them.
What is the 16s rRNA gene?
300
This part of the plasmid is recognized by bacterial host cell replication machinery.
What is origin of replication?
300
This enzyme could be said to be synonymous to the denaturation step of PCR.
What is helicase?
300
A mix of polymerase, buffer, primers and DNA template strand that does not amplify is missing this component.
What is dNTPs?
300
This substance allows visualization of DNA under UV light.
What is SYBR Safe?
300
Some microorganisms use this single tentacle-like organelle to move in its environment.
What is the flagella?
400
Your target location when placing a DNA insert inside of a plasmid.
What is the multiple cloning site?
400
Remarkably, this enzyme uses a gradient of ions to change its conformation, constantly spinning.
What is ATP synthase?
400
During PCR, the DNA template is read in this direction by Taq polymerase.
What is 3' > 5' ?
400
Gel electrophoresis has been prominently used in forensics because of this technique.
What is DNA fingerprinting?
400
Amazingly, some photosynthetic microorganisms can intelligently navigate to where they need to be in order to make ATP.
What is phototaxis?
500
This specific subclass of enzyme is used less common in cloning because it achieves a much lower efficiency for inserting DNA in the plasmid than the other, more commonly used, subclass.
What is a blunt end cutting restriction enzyme?
500
Also termed the "water splitting enzyme," this protein complex is key to plants and energy production.
What is Photosystem II?
500
3'-A overhangs ("sticky ends") were generated by this process to prepare the PCR products for cloning.
What is extension step with DNA polymerase?
500
The band that appears in the negative control lane consists of these.
What is primer dimers?
500
We used this toxic chemical(s) to extract our microorganisms from our sample.