Introduction
Methods
Results
Conclusion/Discuss.
Misc.
100
Deep sea environment with black smoke and high levels of heat?
What are hydrothermal vents?
100
How researchers caused heatshock protein up-regulation in shrimp?
What is exposure to high temperatures
100
Did heatshock protein expression increase or decrease in the coastal shrimp at higher temperatures?
Increased (by 6 hours, it was 43 fold higher than normal)!
100
True or False: There was a higher inducibility of HSP70 in vent shrimp than coastal shrimp.
True!
100
Terminology for a "gene that is constantly transcribed"
What is a constitutive gene?
200
Deep sea shrimp species?
What is Rimicaris exoculata/hydrothermal vent shrimp?
200
DOUBLE JEOPARDY: True or false - do plants express heatshock proteins?
True! Plants, bacteria, archaea, and animals express HSP's.
200
Did the vent shrimp or the coastal shrimp express more heat related genes?
The vent shrimp expressed 4 HSP's
200
True or False: vent shrimp typically encounter sustained temperature close to its thermal limits in situ
False, They rarely encounter sustained temperature close to their thermal limits in situ
200
Terminology for a "gene that is only transcribed as needed"?
What is a facultative gene (such as HSP)?
300
Coastal, warm waters shrimp species?
What is Palaemonetes varians/coastal marsh shrimp?
300
Technique used to amplify DNA sequences?
What is PCR (polymerase chain reaction) amplification?
300
Which specific kinds of heat shock proteins were they looking at?
What is HSP70
300
True or False: low levels of hsp70 gene have been recently reported in natural populations of vent shrimp
True
300
The tissue from which RNA was extracted in the shrimp
What is their abdomen muscles?
400
Stress protein studied in this experiment in shrimp species
What is HSP70?
400
Why was the water temperature for the vent shrimp aquarium higher than for the coastal shrimp?
The vent shrimp tolerated high temps better - coastal shrimp died at too high of temperature.
400
How many isoforms of inducible HSP70 were found in vent shrimp?
Two
400
True or False: vent shrimp may avoid prolonged contacts with high temperature zones through a biochemical response
False, it's actually a behavioral response
400
The constitutive form of heat shock protein 70
What is HSC70?
500
Biological function of heatshock proteins?
What keeps proteins from denaturing under high temperatures/protects cell from stresses
500
Besides temperature, what other facto was altered between the types of shrimp in the ramp-shock experiments?
What is pressure?
500
What kind of indicator is expression of HSP70 used as?
What is bioindicator of stress
500
True or False: a strong molecular response may be triggered in case of occasional and unpredictable thermal extremes
True
500
True or False: The level expression obtained in vent shrimp is comparable to species that rarely undergo heat stress in their habitat.
True
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