A liquid that contains nutrients for the cells to grow in.
What is Luria Broth
100
These short strands of DNA help to select the region of DNA to be amplified.
What are primers.
100
You subtract this number of base pairs from your cut band to approximate insert size.
What is 700bp.
100
This major component of wash buffer can interfere with sequencing reactions.
What is ethanol.
100
This is the name of the organism the program studied genes from before Landoltia punctata.
What is Wolffia australiana.
200
You should pick colonies of this color.
What are white colonies.
200
The name of the enzyme we use to synthesize new DNA strands in PCR reactions.
What is Taq polymerase.
200
We use this type of restriction enzyme in our labs to approximate insert size (include full name).
What is PvuII-HF.
200
This solution denatures the cell membrane of bacterial cells, ultimately lysing the cells.
What is Solution II.
200
According to Ms. Sanelli, you should NOT dress like this to a poster session.
What is like you're working on the street.
300
The duckweed DNA insert disrupts proper expression of this gene on the pTriplEx2 vector, and therefore prevents this protein from being formed (name gene and protein for credit).
What is LacZ, beta-galactosidase.
300
The three phases in a PCR reaction.
What is denaturing, primer annealing, elongation.
300
This restriction enzyme was used to ligate the duckweed DNA insert into the plasmid during cDNA library construction.
What is Sfi
300
The purpose of miniprep is to purify/isolated this.
What is plasmid DNA.
300
These are the names of the 4 WSSP staff members.
What are Dr. Andrew Vershon, Dr. Janet Mead, Mr. John Brick, Ms. Sue Coletta
400
This can occur if the vector re-ligates out of frame without a duckweed insert.
What is a false positive.
400
A student sees that there are two non-primer bands in his lane on a PCR gel. List 2 valid explanations.
What is contamination of one prep or contamination of primer tubes.
400
A student observes 2 bands in the "cut" lane of her restriction digest gel. What has the student likely done to mess up?
What is nothing--the student did not mess up.
400
After you add solution III and centrifuge your tube, your plasmid DNA is in this part of the contents of the tube.
What is the supernatant.
400
The Waksman room was renovated during this year.
What is 2014.
500
The "X" in X-gal is what type of compound?
What is an indole.
500
This individual invented PCR, likely under the influence of a hallucinogenic drug.
What is Kary Mullis
500
Restriction enzymes are found naturally in bacteria, which use the enzymes to cut up foreign DNA that may be harmful. What enzymes carry out a process that protects bacterial genomic DNA from the bacteria's own restriction enzymes?
What are methylases.
500
This is the name of the process used to transfer plasmid DNA from the spin column to another buffer of sterile water.