Pick Your Poison
Wonka's World
Would you like fries with that?
A"fection"?
Sesame Street
100
Used to treat for mycoplasm in cells
What is ciproflaxin
100
Terminal processing of these N-glyc is necessary (and sufficient) for apical delivery of Endolyn
What is N-glyc at positions 68 and 74
100
Current IDT PO number
What is 929609
100
This procedure causes issues with the gate function of tight junctions
What is Amaxa electroporation
100
HA hybridoma antibody
What is FC125
200
Concentration of G418 we use for most stables
What is 0.4mg/ml
200
Mannosidase I inhibitor
What is kifunensine (also a weak class II inhib)
200
What is the best way to get Jenn the information on items that need to be ordered
What is the PRISM sheet on the database
200
Optimal length for siRNA oligos
What is 21-25bp (length that dicer enzyme cuts dsRNA to)
200
Cell line that stably expresses TA and pIgR
What is T23
300
Neurotoxin used in SDS-Page gels
What is acrylamide
300
Inhibits synthesis of new N-glycans
What is tunicamycin
300
Information that should be written by the opener on every packing slip (3 things)
What is date, your name, where the item was put away
300
UV wavelength we use to determine virus concentration
What is 260
300
Monoclonal VSVG hybridoma
What is 8G5
400
Drug resistance of mApple constructs
What is kanamycin
400
Addition of GalNAc to serine or threonine residues
What is O-glycosylation
400
Two items that need order forms other than the Prism one
What are CO2 tanks (Valley National Gas) and 95% Ethanol (Pharmco)
400
ul of endolyn virus needed to infect 6x12 wells of T23 cells at an moi of 100 (assume 1.7ul/well for moi of 250)
What is 4.08ul
400
Base media for CCD cells
What is DME-F12
500
Chemical used in biotinylation protocol that is on the government's 'chemical watch list"
What is TEA
500
Our DME has "high" levels of this
What is glucose
500
What information should be typed into each order form before it goes to Jenn (4 things)
What is item name, catalog number, quantity and price - Cheryl needs $ now with new system
500
Enables easy seperation of sense and antisense siRNA so antisense can be incorporated into RISC complex
What is Sense 3' mismatch
500
Our amphibious cell line
What is A6
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