In this field, DNA can be analyzed directly from environmental samples
Metagenomics
100
In what year did Rosalind Franklin discover Photograph 51?
1952
100
In this model of replication, fragments of the original DNA molecule serve as templates for assembling two new molecules, each containing old and new parts, perhaps at random.
Dispersive
100
Transcription begins at the __________
iniation site
100
_________ & ___________ form myofibrils, which are bundled together to form muscles
Myosin & actin
200
The field of __________ was developed to analyze
DNA sequences using sophisticated mathematics and com-
puter programs to handle the large amounts of data generated in genome sequencing.
bioinformatics
200
T & C are pyrimidines. True or false?
True
200
In eukaryotic replication, replication forks move ______ one another
Away from
200
The four ribonucleoside triphosphates are ATP, GTP, CTP, and
TTP.
True or false?
False, UTP
200
Gel electrophoresis uses a gel as a ________ to separate nucleic acids by size and electrical charge
molecular sieve
300
The materials needed for PCR are:
DNA sample
Primers
Nucleotides
Polymerase
PCR machine
300
________ is an enzyme's ability to catalyze "consecutive reactions without releasing its substrate".
Processivity
300
_________rapidly hydrolyzes released pyrophosphate into two phosphate molecules.
Pyrophosphatase
300
____________ help direct the polymerase onto the pro-
moter.
sigma or transcription factors
300
Whats the meaning for the acronym PAGE
Polyacrymylide gel electrophoresis
400
In what year did Sanger win the nobel prize for his fragmentation method?
What is 1977
400
The ________ ensures the protective nature of the DNA pol
Sliding clamp
400
During RNA primer removal, the final ribonucleotide is removed by a ___________?
5’ exonuclease
400
DNA is locally unwound by __________ to serve as a template for RNA synthesis
RNA polymerase
400
When performing our gel lab, we
1. Denatured our protein
2. Extracted our protein
3. Ran our protein on an SDS PAGE gel
True or false?
False. You extract your protein, then denature it.
500
Archea has fewer genes than bacteria. True or False?
False
500
On average, DNA polymerase inserts one incorrect nucleotide for every _______ nucleotides added
10^5
500
DNA synthesis is initiated when the 2’-OH mediates the nucleophilic attack of the phosphate of the incoming dNTP.
True or false?