Microarray
Pulse Chase
embryonic stem cell development
Diabetes
Hodge Podge
100
A microarray is made up of many different
What is DNA sequences
100
The purpose of the pulse chase experiment is to examine...
What is cellular processes that take place over time?
100
From day 1-4 an embryo is referred to as a ___________ and its potency is ___________.
What is morula and totipotent?
100
One major diagnostic symptom of diabetes is the presence of
What is high levels of glucose in the blood?
100
A chimera is...
A Chimeric mice are important tools in biological research, as they allow the investigation of a variety of biological questions in an animal that has two distinct genetic pools within it. These include insights into such problems as the tissue specific requirements of a gene, cell lineage, and cell potential.
200
A specific genomic sequence that corresponds to a unit of inheritance is know as a..
What is a gene?
200
Which term best describes the heavy and light isotopes of nitrogen used to determine the structure of replicated DNA.
What is a label?
200
From day 14-21 an embryo is referred to as a ___________ and its potency is ___________.
What is gastrula and it multipotent?
200
The immune system attacks and destroys beta cells in which type of diabetes?
What is type 1 diabetes?
200
There is a risk of developing a teratoma when __________ cells are implanted in the body.
What are undifferentiated stem cells?
300
A spot that fluoresces on a microarray indicates that a gene is
What is expressed?
300
Describe the pulse chase experiment conducted to determine the structure of the replicated strands of dna. Describe the label and the result (chase).
What is using a heavy isotope of nitrogen in a strand of dna and culturing it in a petri dish with a lighter strand of dna.
300
From day 5-14 an embryo is referred to as a ___________ and its potency is ___________.
What is bastula (blastocyte) and is pluripotent?
300
Which symptom is a characteristic of type 2 diabetes?
What is an individual becomes insulin resistant?
300
Describe the process of nuclear transfer and what it would be used for.
What is removing a nucleus from an egg cell and injected the nucleus from the individual that will be receiving the stem cells from it. ...cloning
400
What molecule serves as the template for synthesizing the cDNA that is hybridized to a microarray? The cDNA sample used in a typical microarray experiment is made from ________
What is mRNA? (Because DNA is more stable. The mRNA indicates the that the gene is being expressed)
400
Describe the results of the pulse chase experiment conducted on the beta cells of the pancreas. What were scientists trying to determine?
What is whether the beta cells were produced through mitosis or adult stem cells of the pancreas?
400
List the three tissue layers formed during gastrulation
What are ectoderm mesoderm endoderm
400
Describe what happens in the body when blood sugar is low in order to return to homeostasis. Use the terms: glucagon, glycogen, glucose, liver and pancreas.
What is the pancreas releases glucagon, which stimulates the breakdown of glycogen into glucose in the liver, raising the amount of sugar in the blood.
400
What are three disadvantages of using adult stem cells?
What are limited cell types, not found in all tissues, difficult to isolate, difficult to maintain, difficult to grow in the laboratory?
500
What is the function of a microarray experiment in the context of stem cell research?
The microarray enables scientists to determine which genes are turned on in certain cells and can lead to an understanding of which genes cause differentiation.
500
Why were the results of the pulse chase experiment on the pancreas a set back for stem cell researchers working on beta cells?
What is it determined that stem cells were not involved in the production of new beta cells. Ruling out using adult stem cells as a future therapy.
500
What do the tissues formed in gastrulation differentiate into?
What are ectoderm = skin and nervous system, mesoderm = muscles, bones, and heart. endoderm = inner organs, (gut),
500
Describe what happens in the body when blood sugar is high in order to return to homeostasis. Use the terms: glycogen, glucose, insulin, tissues, liver and pancreas.
What is high blood sugar promotes insulin release in the pancreas which causes glucose to be stored as glycogen in the liver and causes glucose to be taken up from the blood into the tissues.
500
Describe an induced pluripotent stem cell
What is an adult stem cell that has been given growth factors, transcription factors...signals to get them to go backwards and become pluripotent rather than multipotent.
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