400
Biochemical reactions are consistent with Salmonella. A suspension is tested in polyvalent antiserum A through G and Vi antiserum. There is agglutination in the Vi antiserum only. What should be done next?
A. Boil suspension for 10 min
B. Test with individual antisera for agglutination
C. Report "no Salmonella isolated"
D. repeat biochemical ID of organism
What is A. Boil suspension for 10 minutes to inactivate the heat labile Vi antigen, then appropriate agglutination can take place