OBBN Program
Study Design
Sampling Methods
Diagnostic Characteristics
Miscellaneous
100

What is biomonitoring?

assessment using biological indicators

100

Reference sites are sites that are described as having...

Minimal human impact

100

What is the name of the sampling equipment and method used for streams?

Traveling kick and sweep with a D-net

100

Suckers at both the anterior and posterior ends are a diagnostic feature of which group?

Leeches (Hirudinea)

100

What is the standard mesh size for sampling nets?

500 microns

200

The OBBN program provides sampling protocols for which types of aquatic habitat?

Lakes, streams and wetlands


200

When collecting multiple samples in a stream, should they be collected from upstream to downstream, or downstream to upstream?

Downstream to upstream

200

What is the protocol for time and distance for a traveling kick-and-sweep?

10 m distance in 3 minutes

200

What is the key diagnostic difference between mayflies and stoneflies?

Mayflies have a single tarsal claw

200
What are two disadvantages of using formalin to preserve samples?
Dissolves calcareous shells and human health concerns (i.e. carcinogenic)
300

Why use benthic invertebrates are bioindicators?

1) they are easy to sample, 2) the equipment is inexpensive, 3) their pollution tolerances are well documented

300

What is the Reference Condition Approach?

The use of multiple minimally impacted reference sites to define the normal range of biological conditions at a test site
300

What are the two recommended methods for randomized sub-sampling?

Marchant box and teaspoon method

300

Forewings modified as hardened plates or “elytra” are diagnostic of which group?

Beetles (Coleoptera)

300

What are three essential features to include on an OBBN field sheet site sketch?

Sampling locations, north arrow and access roads

400

What are the objectives of the OBBN program?

Evaluate aquatic ecosystem conditions, provide a biological complement to chemical monitoring, measure effectiveness of programs, support development of biocriteria

400

What is a disadvantage of the BACI study design?

BACI requires samples to be collected before any stressor exposure or biological effect, and such samples are rarely available

400

How many benthic invertebrates should be picked from each replicate/sub-sample?

At least 100
400

Membranous wings, 6 legs and sucking mouthparts are diagnostic features of which group?

True Bugs (Hemiptera)

400

Why is it important to sieve your samples?

Remove fines and organic material

500

Which agency is the lead for OBBN, and what is their role?

MECP is the lead; their role is to coordinate research, and provide technical advice and equipment to OBBN partners

500

Why does the OBBN protocol recommend using multiple indices in a bioassessment?

Different indices emphasize the multiple different aspects of benthic invertebrate data

500

What are the sampling units for streams, wetlands, and lakes?

Meander wavelength, wetland segment, and lake segment

500

What are four diagnostic features of chironomids?

Sclerotized head, anterior and posterior parapods, J-shaped, colour variable (red, white, green, yellowish)

500

List five situations where you should not enter a waterbody to collect benthic invertebrate samples.

Dangerous flow/current, private property, sensitive habitat/spawning area, adverse weather conditions,

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