Factors Affecting Enzyme Rates
Activation Energy
General Enzyme Knowledge
Enzyme-Substrate
Catalase Lab
100
If the concentration of enzymes is increased, what happens to the rate of the reaction?
The reaction rate will increase.
100
The energy needed to start a reaction is called this.
What is activation energy?
100
Enzymes _________ reactions in the cells.
What is speed up?
100
This is the name of the model that describes how a substrate fits with an enzyme.
Lock and Key
100
This was the substrate used in the lab.
Hydrogen Peroxide
200
What does "Competition for Active Site" mean? How does it affect the enzyme rate?
Another other molecule sits in the active site, blocking the substrate from reacting with the enzyme. Rate is reduced.
200
True or False: All reactions require the same amount of ENERGY to get started.
What is false?
200
Enzymes are this type of macromolecule.
What is proteins?
200
This is what the Enzyme acts upon.
What is a substrate?
200
When acetic acid was added to the liver, the rate of the reaction _______.
Decreased.
300
The 4 factors affecting enzyme rate.
Enzyme Concentration, Substrate Concentration, Change in pH and temp., Competition for Active Site
300
This protein will reduce the activation energy.
What is an enzyme?
300
Enzymes contain these elements.
C,H, O, N
300
Where the specific substrate fits in the enzyme.
What is an active site?
300
Why could liver be used in the lab even though it came from a dead animal?
The enzyme can remain intact (active) for long periods of time as long as it is refrigerated.
400
This is the term for when an enzyme's active site changes shape due to a pH or temperature far from optimal.
Denatured
400
Enzymes lowers activation energy & speeds something up. Another word for this is a ____________.
What is catalyst?
400
True or False: Enzymes are used up during a reaction (not reusable)
False
400
The “new” substance created during reaction.
What is a product?
400
Was the catalase reusable? What proved this?
A reaction occurred every time new substrate (hydrogen peroxide) was added.
500
What is the optimal pH for the enzymes pepsin and trypsin?
Pepsin: 2.5-3
Trypsin: 7.5-8
500
This number on the graph represents the activation energy for the enzyme
What is 1
500
For each enzyme, there is a _________ substrate.
Specific
500
After the reaction, the enzyme is _________.
What is reusable?
500
This is why there was no reaction after the liver was heated in the hot water bath.
The enzymes became denatured (changed shape) and the substrate could not bond to the active site