Edman Degradation
PTH that absorbs at ...
What is 254 nm
Trypsin and Pepsin are examples of
enzymatic cleavages
Separates molecules based on their
What is Mass-to-Charge (m/z) ratio
Angle about the C(α)-C bond is denoted
Angle about the C(α)-N bond is denoted
What is psi and phi
What is considered to be a helix breaker
What is proline
In the sequence
N - KPTRAG - C
the first amino acid identified
What is Lysine
What are 3 (any) of the 5 enzymes that cleave?
What is Trypsin, V8 Proteases, Chymotrypsin, Pepsin, Thermolysin
Ion X has a charge of 35, what is the charge of ion Z in the same series?
(Look at chart at front of classroom)
What is 37
Forbidden angle combinations
(there are 3)
What is 0 and 180; 0 and 0; 180 and 0
alpha helix net dipole movement
What is partial positive at the amino end and partial negative at the carboxyl end
Edman reagent (phenyl isothiocyanate) reacts with
What is the N-terminus of the peptide
BNPS-Skatole (chemical cleavage) cleaves after which amino acid(s)
What is Trp (Tryptophan)
What is 0.25 m/z
Amino acid with the widest range of angles
What is Glycine
What is anti-parallel
A sequencing method that uses the C-terminal amino acid
What is Carboxypeptidases
Enzyme that cleaves after Phe, Trp, Tyr, Leu.
What is Chymotrypsin
A mass spec is obtained and the m/z are 685.9 and 571.3. What is the charge (z) of the molecule.
(assume the peaks are next to each other)
What is 5
Length of a peptide bond
What is 1.33 Angstroms
Reason for entropy gain in quaternary protein structures
(favorable/less favorable)
What is because of the burying of hydrophobic groups. Favorable
The four steps prior to the sequencing of peptides
What is
1. separate non-covalently linked chains
2. break and block disulfide bonds
3. cleave proteins into peptides
4. isolate and separate peptides
Chymotrypsin cleaves the sequence
RLNGFKTWPSC
How many strands are created
What is 3 total strands
A mass spec is obtained and the m/z are 735 and 673. What is the mass (m) of the molecule.
(assume the peaks are next to each other)
What is 7955.6
Describe a Ramachandran Plot for a peptide
(What do the colored portions mean, what do the uncolored portions mean)
What is, the colored regions show the phi and psi values that are allowed while unshaded regions are where it is not allowed.
Collagen is made up of
(structure not amino acids)
What is a right-handed triple helix composed of three left-handed helical chains