RANDOM
When the change in free energy in a reaction decreases (negative), the reaction will be ______?
Exergonic
T or F: The buffering capacity of a good buffer allows about 10 times more acid or base to be added than the pK a and maintain close to the same pH.
True
A triprotic acid has _____ pKas associated with it.
3
In a water molecule, where would you find a slightly more positive charge?
on the hydrogens
Sephadex is used in which type of chromatography?
Size exclusion (SEC) chromatography
In a reaction ΔG is greater than zero, so this means that the reaction will be _____ and _______?
Non-spontaneous and Endergonic
What is the pH of a solution with the concentration of 1x10^-9 M NaOH?
5
What are the amino acids that are acidic?
Aspartic acid
Glutamic acid
T or F: A miscele is an example of an amphipathic molecule.
true
Proteins move through SDS-PAGE gels by way of (a/an)
electric field
True or False: Enzymes are catalysts that inhibit reactions?
False
What is the pH of a buffer solution of acetic acid and acetate ion with a pKa = 4.86, when 10 mol of acetic acid is titrated with 6 mol of sodium hydroxide?
5.04
What are the amino acids that are basic?
Histidine
Lysine
Arginine
T/F Cholesterol is an example of a hydrophilic molecule.
false
Ion chromatography is one way to separate proteins based on __________________. Cation
exchange columns are meant to bind _____________ moieties.
charge, positive
The backbone of a protein is made up of which chemical groups
amino group and carboxylate group
What is the pH of a solution with the concentration of 1x10^-4 M HCl?
4
What are the amino acids that are polar?
Serine
Threonine
Tyrosine
Cysteine
Asparagine
Glutamine
Disulphide bonds are examples of which type of bond or interaction?
salt-bridge/ covalent
The theory behind SDS-PAGE gels is that proteins are separated based on
Size (smaller at the bottom; larger at the top)
what are you doing this weekend?
:D
Consider a solution of sodium phosphate and disodium phosphate, in which the concentrations are [HPO4^2-] = 0.067 M and [H2PO^4-] = 0.17 M, with pKa of 7.42. If 1.0 mL of 0.10 M HCl is added to 99.0 mL of buffer, what is the resulting pH of the buffer solution?
7.01
what are the amino acids that are non polar?
Alanine
Valine
Leucine
Isoleucine
Proline
Methionine
Phenylalanine
Tryptophan
Order the following types of bonds or interactions from least strength (1) to most strength (5):
Ionic interactions
Van der Waals interactions
Hydrogen Bonds
Covalent bonds between oxygen and hydrogen
Covalent bonds between carbon and hydrogen
(1)
van der waals interactions
hydrogen bonds
ionic interactions
covalent bonds between carbon and hydrogen
covalent bonds between oxygen and hydrogen
(5)
If a protein was excreted into the cytoplasm of the cell, what order of steps would need to be
completed in order to isolate the protein of interest?
Homogenization, differential centrifugation, chromatography