DNA Replication
what are the 3 enzymes you need to know for DNA replication?
topoisomerase, DNA polymerase, and helicase
what are the 3 parts of transcription?
initiation, elongation, termination
what are the 3 parts of translation?
initiation, elongation, termination
the 3 phases of interphase
G1, S, G2
during telophase, we call the DNA ____________
(chromosomes, sister chromatids, daughter chromosomes, etc)
daughter chromosomes
what are the 4 requirements of the genetic material?
information, transmission, replication, variation
name one difference between DNA polymerase and RNA polymerase
uses dNTPs vs. rNTPs
all are template dependent vs. most are template dependent
requires a primer vs. doesn't require a primer
proofreads vs. doesn't proofread
what types of RNA are required for translation?
mRNA, tRNA, rRNA
how many daughter cells are made from mitosis?
2
how many daughter cells are made from meiosis?
4
name one difference between prokaryotic (bacteria) and eukaryotic DNA replication
circular DNA vs. linear DNA
one chromosome vs. many chromosomes
one origin of replication vs. multiple per chromosome
no chromosome ends vs. end of chromosome issues
the template strand is
5' AGGTCCCAGT 3'
What is the sequence of the mRNA?
3' UCCAGGGUCA 5'
what is the sequence of the start codon?
**bonus** which amino acid does it code for?
AUG
Methionine
name the steps of mitosis in order
prophase
prometaphase
metaphase
anaphase
telophase
cytokinesis
during prophase, what happens to chromosomes/sister chromatids?
they condense to make heterochromatin
There are ___ (#) replication forks at each origin of replication
2
what happens during transcriptional termination?
stuff
the _________ acts as a platform for transcription to take place
small ribosomal subunit
there is one checkpoint during mitosis. what is it and when does it occur?
during metaphase, are microtubules attached to sister chromatids from both poles?
tetrad definition that he gave
homologous pairs of sister chromatids
what are Chargaff's rules?
A+G=T+C
A=T and G=C
the sequence of RNA that is synthesized is identical (except for U vs. T) to the _____ strand of the DNA
coding
what happens during termination of translation?
stop codon, release factor comes in, everything dissociates
what are the 3 types of microtubules
kinetochore microtubules
polar microtubules
astral microtubules
meiosis I is a ________ division
reduction division