The Fundamentals
Block diagram it
So much spectroscopy
We got that here!!
More techniques
100

It's a charged particle and it sure as hell not positive.

Electron

100

Mass analyzers need this but the other instruments we discussed in class don't. I'm pretty sure Jordin Sparks has some song related to this.

Vacuum

100

Absorbance is equal to molar absorptivity multiplied by pathlength multiplied by concentration. You can also drink this if you're over 21.

Beer's Law

100

It's the most useful type of LC. At least according to Mark. All of you except Julia will probably disagree.

FPLC

100

You can use this with any molecules. Majority of protein structures were determined with this technique. Dr. Guillet got us a new one for single crystals!

X-Ray

200

One would guess that this is Xiao's favorite molecule. We could be wrong though.

Caffeine

200

We don't want wonky signals just because the magnetic field strength is not uniform across our sample. Good thing we can do this!

Shim

200

Instead of radiation being emitted from a singlet state, it is emitted from a triplet state. Sounds forbidden to me.

Phosphorescence

200

Full name for this 'fancy' flame test. Ro was definitely fired up when we, well, fired it up.

Atomic Absorption Spectroscopy

200

The "M" in CryoEM, a very popular technique for structural biologists lately.

Microscopy

300

Not instrumental. Gravimetric analysis, for instance.

Classical

300

It's a transmitter! No, it's a receiver! No! Yes? Hold on. It's both?

Tranceiver

300

Two light sources that are most commonly used for UV-Vis spectroscopy. One of them is quite noble, the other is in heavy water.

Xenon/Deuterium

300

We call it a "300 MHz" even though "MHz" is not the unit for magnetic field strength.

NMR

300

ITC stands for Isothermal "T" Calorimetry. You can quantify the interaction of A and B based on the heat released as you add a little bit of A into B. Adding a little bit of something to another thing... this has to remind you of someThing...

Titration

400

Doing an external calibration curve is not always the best. In this case, you spike your sample with a stock solution of known concentration.

Standard Addition

400

GC detector. Perfect for polychlorinated biphenyls or PCBs, highly carcinogenic compounds now banned in the US.

Electron Capture Detector

400

One photon, SO MANY electrons. That'll surely boost your signal!

Photomultiplier Tubes

400
You can easily tell what this is because the detector is at 90 degrees from the incident light.

Fluorometer/Spectrofluorometer

400

That's some fancy centrifuge. I heard it can even tell if a protein is changing conformation.

Analytical Ultracentrifugation

500

Time to frequency, frequency to time. From NMR to orbitrap.

Fourier Transform

500

It's not an absorption filter. This one has higher transmittance and a narrow bandwidth!

Interference Filter

500

Sshhh! Too bad ssshhh-ing does not remove random voltage fluctuations or we can easily get rid of this noise.

Thermal/Johnson/White Noise

500

Rayleigh is for rainbows. This one is pretty 'Stokes.'

Raman

500

Garbage in, garbage out. Arguably, it's the most important part of your experiment. It doesn't really matter if you have the fanciest instrument. If this is garbage, your data will be garbage.

Sample

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