It is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution.
SPECTROPHOTOMETRY
Give one application of spectrophotometry
•Forensic sciences
• Molecular biology
• In disease diagnosis/ pathological states (changes)
• Blood (the blood plasma, and the formed elements
• To identify and quantify biological molecules
• Measurement of concentration
• Detection of impurities
• Elucidation of the structure of organic compounds
• Chemical kinetics
It is a measurement of light that absorb by the sample.
Absorbance
Who invented spectrophotometer
ARNOLD J. BECKMAN
What company Beckman had started in 1935, where they invent the spectrophotometry
National Technologies Laboratories
Give all the uses of spectrophotometry
Measure the concentration of a solution
Identify organic compounds by determining the absorption in maximum
Used for color determination within the spectral range