EASY
AVERAGE
DIFFICULT
5

It is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution.

SPECTROPHOTOMETRY

5

Give one application of spectrophotometry

•Forensic sciences

• Molecular biology

• In disease diagnosis/ pathological states (changes)

• Blood (the blood plasma, and the formed elements

 • To identify and quantify biological molecules

 • Measurement of concentration   

• Detection of impurities 

• Elucidation of the structure of organic compounds 

• Chemical kinetics 

5

It is a measurement of light that absorb by the sample.

Absorbance

15

Who invented spectrophotometer

ARNOLD J. BECKMAN

15

What company Beckman had started in 1935, where they invent the spectrophotometry

National Technologies Laboratories

15

Give all the uses of spectrophotometry

Measure the concentration of a solution 

Identify organic compounds by determining the absorption in maximum 

Used for color determination within the spectral range

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