Factor Theory
Factors VIII, IX, XI,XII,are in this pathway of the coagulation cascade.
What is the Intrinsic pathway?
Cause for rejection when transporting samples for Factor Assay testing on ice.
What is activating Factor VII and loss of vWF.
Recommended concentrations for deficient plasma reagents include a certain minimum percentage of the Factor being tested and of the other Factors.
What is < 1% for testing Factor and all other Factors > 50%
Name the three types of calibration curves used in Factor Assays.
What are linear, polynomial, and low curves?
In the SCE this test calculates the mean of the 1/10, 1/20 and 1/40 dilutions for Factor 8.
What is the F8M?
These pathways in the coagulation cascade includes Factors II, V, VII and X
What is the Extrinsic and Common pathways?
CLSI recommendation for thawing frozen samples for Factor Assays
What is a rapid thaw in 37 degree waterbath, 5min/ml?
When raw data values exceeds the limits of the curve.
What is an extrapolated value.
True or False? The low curve calibrator value must be manually calculated and entered into the Instrument software.
What is False?
Remember this instrument setting in the Factor methodology may need to be adjusted when using SCE Factor Rules.
What is remove auto redilute conditions from setup on analyzer.
The mixture of a diluted sample with a deficient plasma with a single step of recalcification leading to clot formation is known as
What is a one-stage Clotting Factor Assay?
This cutoff is used to determine the sensitivity of the PT and PTT reagents in Factor Assay testing when the PT and PTT results are prolonged.
What is < 30% activity?
This requirement proves the validity of the Factor assay when the patient sample and calibrator both behave in a similar way under the same test conditions.
What is Parallelism?
This statistical value of a calibration curve represents how well points fall on the curve.
What is the R value?
In the SCE, this is where you can set up a battery of tests to run Factor dilutions. Example, 1/10, 1/20, 1/40.
What is in the General Tab of The Test?
To diagnosis patients presenting with a prolonged PT or PTT to rule out common conditions such as deficiencies ,inhibitors or persistent hereditary evaluation ( requires confirmation by family studies) that may predispose a patient to thrombosis.
When to perform a Factor Assay?
In Factor testing, this number of sample dilutions is recommended to rule out inhibitors and improve precision of the assay.
What is 3 dilutions?
This methodology makes Factor assays exempt from CAP AMR verification.
What is clot based?
Given the results of the calibration points a line is drawn that connects each individual point.
What is a polynomial curve?
This Factor test in the SCE is used to send the final result to the LIS when using Factor rules.
What is the Roman numeral test?
Although these coagulation proteins do not cause bleeding their activity can be tested using a PTT based Factor Assay
What are HMW Kininogen and Prekallikrein?
This Factor Inhibitor inhibits complete factor activity.
What is Type I Inhibitor or Alloantibodies?
These Inhibitors can inhibit partial factor activity or can present as nonparallelism.
What is Lupus Anticoagulant and Anticoagulants?
The set of operations that establish under specified conditions, the relationship between reagent/instrument system response and the corresponding concentration/activity values of an analyte.
What is a calibration?
This is where the tests are pulled from the SCE database.
What is Paramsinstall?