DNA & Replication
These proteins keep DNA open during replication.
What are single-stranded binding proteins?
Under specific media conditions, you observe that a transcriptional activator binds upstream of your promoter on the DNA. However, you still don't see downstream transcription of the operon. What is a possible cause of this?
Transcriptional repressor, RNA polymerase degraded, Sigma factors being sequestered/degraded, etc.
This level of protein structural complexity describes an oligomeric complex of 12 proteins interacting together.
What is quaternary?
An atom with weak electronegativity and an atom with strong electronegativity will likely form this type of covalent bond.
What is a polar, covalent bond?
These scientists won a Nobel prize for discovering the lac operon. (name one, last name is fine)
Who are Jacob and Monad?
You would get rid of this part of a nucleotide to block it from being added to a newly synthesized DNA strand.
What is the 5' phosphate or the 3' hydroxyl?
These two base pairs are most commonly found in a promoter sequence.
What are adenine and thymine?
What is a release factor?
Two molecules share these within a covalent bond.
What are electrons?
This person holds the current record for most hotdogs eaten in 10 minutes. (Bonus for how many hotdogs he ate)
Who is Joey Chesnut (75 hotdogs).
Name two things that are able to unwind a DNA strand (in any context)
What are helicase, RNA polymerase, and heat.
This enzyme converts RNA to DNA.
What is reverse transcriptase?
These molecules initiate translation.
What is the translation initiation complex (small ribosomal subunit, initiator methionine tRNA)
True or false: a cytosolic protein has the potential to be soluble in Kool-Aide (explain why or why not based on Kool-Aide as a buffer).
What is true because cytosolic proteins are typically soluble in polar solvents, and Kool-Aide would be a polar solvent because it is water-based.
The song "Ashes" from the hit Marvel movie Deadpool 2 is sung by this Canadian artist.
Who is Celine Dion?
Describe the difference between eukaryotic and prokaryotic nucleotides.
What is there is no difference?
We delete a portion of a genome that does not code for a protein, but does contain a promoter sequence - the resulting organism dies. This region likely encoded ___.
What is rRNA or tRNA? (Or any RNA besides mRNA)
You want to have a gene transcribed but not translated. You could mutate this to block translation of this one specific gene.
What is the ribosomal binding site?
This is a weak bond that is based on the interaction between two polar molecules.
What is a hydrogen bond?
A chef's hat contains exactly this many pleats.
Eukaryotic replication and prokaryotic replication are different in that they occur in these locations for each.
What is nuclear vs cytosolic?
This biomolecule is used as a template for both Replication and Transcription.
What is DNA?
A motif is this level of protein structural complexity.
What is supersecondary?
What aspect of carbon makes it so useful in organic chemistry?
What is four valence electrons?
This animal is the only animal in existence that produces the necessary ingredients to make a fluffy omelet.
What is a platypus?