DNA Replication
The only DNA polymerase in E. coli with a 5' to 3' exonuclease activity, this enzyme also plays a role in repairing damaged DNA.
What is DNA polymerase I?
The initiation of transcription in E. coli requires this protein factor, which joins the RNA polymerase core enzyme and binds to the -10 and -35 regions of the promoter.
What is the sigma factor (or subunit)?
These sulfur containing amino acids play a critical role in the initiation of translation or the formation of disulfide bonds during the protein folding process.
What are methionine and cysteine?
These amino acid residues are rarely found in elements of secondary structure, but frequently occur in turns and loops connecting elements of secondary structure to each other.
What are glycine and proline?
Nucleic acids are commonly quantitated by measuring the absorbance at this wavelength, which is due to the aromatic properties of the nitrogenous bases.
What is 260 nm?
This protein factor increases the processivity of DNA polymerase III from ~12 nucleotides to greater than 5000 nucleotides and also increases the rate of polymerization.
What is the sliding clamp (or beta clamp)?
The sequence of RNA (in the 5' to 3' direction) that would be produced by a sense strand of DNA with the sequence ATGCCGATC (also in the 5' to 3' direction) after transcription.
What is AUGCCGAUC?
The only amino acid in the following list that does not have a nonpolar side chain: alanine, valine, leucine, phenylalanine, glycine, glutamine, isoleucine, proline
What is glutamine?
An alpha helix that consists of 18 amino acid residues and and has a length of 27 angstroms would have this number of complete helical turns.
What is 5?
According to University Prime Time, the only beer on this list that is not ranked as one of the ten most popular college beers: Bud Light, Bush Light, Natural Ice, Samuel Adams, Miller Lite
What is Samuel Adams?
This E. coli topoisomerase is responsible for relieving torsional strain ahead of the replication fork generated by helicase and is essential for DNA replication.
What is gyrase?
The phosphorylation status of this protein domain is crucial to the initiation, elongation, and termination phases of RNA polymerase II mediated transcription.
What is the CTD (C-terminal domain of Rpb1)?
The net charge of the predominant form of the polypeptide Met-Arg-Cys-Gly-Ala-Ala-Lys-Ser-Ala-Ala-Gly-Gly at a pH of 7.5
What is +2?
Of the following amino acid substitutions, the one that would be most likely to disrupt the hydrophobic effect and destabilize protein structure: Arg to Lys, Ala to His, Ile to Gly, His to Gln, Asp to Lys
What is Ala to His?
Proteins containing at least one of these two aromatic amino acids can be quantitated using Beer's law after measuring the absorbance at 280 nm.
What are tryptophan and tyrosine?
The number of RNA primers that would be required to synthesize the leading strand of a 30 kb linear segment of DNA with 150 origins of replication
What is 300?
Termination of transcription in E. coli, in many cases, relies on the helicase activity of this protein factor, which separates the mRNA from the template DNA to block further elongation.
What is the rho factor (or rho)?
This type of chromatography is commonly used in LC-MS to separate peptides based on their polarity and typically involves the use of a column with a C18 stationary phase.
What is reversed-phase chromatography (or hydrophobic interaction chromatography)?
This type of secondary structure consists of two or more polypeptide strands that extend in the same direction from N- to C-terminus and are linked by "distorted" or offset hydrogen bonds.
What is a parallel beta sheet?
According to the Los Angeles Times domestic beer power rankings, this "silver bullet" college beer is highly drinkable, with a chuggability score of 4.0.
What is Coors Light?
This eukaryotic DNA polymerase associates with primase, lacks a 3' to 5' exonuclease activity, and is involved in the initiation of DNA replication.
What is DNA polymerase alpha?
This eukaryotic general transcription factor has a helicase activity that unwinds the DNA at the transcription start site and a kinase activity that plays a critical role in promoter clearance.
What is TFIIH?
The pKa value of this amino acid side chain in its free form is ~8.4, but it can range from ~5 to ~12.5 depending on the protein microenivornment the side chain is found within.
What is cysteine?
This general class of proteins is rich in polar and charged residues, lacks defined structure, and can become ordered upon binding to another protein.
What is an intrinsically disordered protein?
Commonly viewed as the hipster college brew, this relatively malty (and skunky) beer got its name from the blue ribbon tied around the bottle neck between 1882 and 1916.
What is Pabst Blue Ribbon (PBR)?