Lac Operon & Reporters
This compound turns blue when cleaved by β-galactosidase.
What is X-gal?
This process allows bacteria to communicate based on population density.
What is quorum sensing?
This fusion tag is commonly used to purify proteins.
What is Glutathione-S-transferase (GST)?
These are advantages of using C. elegans (name one).
What are transparency / short lifecycle / simple genetics?
This index measures movement toward or away from stimuli.
What is the chemotactic index?
This enzyme breaks down lactose into glucose and galactose.
What is β-galactosidase (b-gal)?
This signaling molecule is the signaling molecule we focused on bacteria communication.
What is AI-1 (autoinducer-1)?
GST binds specifically to this molecule during purification.
What is glutathione?
This is what C. elegans eat.
What is bacteria (e.g., E. coli)?
A positive chemotactic index indicates this behavior.
What is movement toward a stimulus?
This gene encodes β-galactosidase.
What is LacZ?
These proteins detect signaling molecules in quorum sensing.
What are receptors?
This is the main reason scientists purify proteins.
What is to study structure/function or isolate a specific protein?
How do C. elegans reproduce?
What is hermaphroditic self-fertilization (and males)?
These structures are used for sensing in C. elegans.
What are cilia?
Why is LacZ commonly used in experiments?
it is reporter gene to measure gene expression
These molecules bind receptors to trigger signaling pathways.
What are ligands?
What type of chromatography is used with GST-tagged proteins?
What is affinity chromatography?
Approximate size of C. elegans.
What is ~1 mm?
How are Tetrahymena cilia primarily used?
What is locomotion?
Name one process regulated alongside bioluminescence.
What is biofilm formation / virulence / secretion?
What happens when quorum sensing reaches threshold levels?
What is coordinated gene expression (e.g., virulence, light production)?
Key difference between C. elegans and Tetrahymena cilia.
What is sensory (C. elegans) vs motile (Tetrahymena)?