Module 1: Review
Module 2: Nucleic Acids
Module 3: DNA Technology
Module 4 + 6: Proteins and Enzymes
Module 5: Protein Technology + Bioinformatics
100
Are often multi-cellular, contain membrane bound organelles, and often have higher orders of organization.
What is a eukaryote?
100
The missing component in a nucleotide that has a nitrogenous base and a ribose (or deoxyribose) sugar unit.
What is a phosphate?
100
Is a small circular piece of DNA that naturally occur in bacteria, that are often used in genetic engineering methods.
What is a plasmid?
100
The monomeric unit of proteins.
What is an amino acid?
100
An algorithm program that aligns several proteins in order to maximize their regions of similarity; showing which amino acids are conserved among the different proteins.
What is a multiple alignment (or Cobalt alignment)?
200
A non-covalent interaction that involves two polar molecules, but neither of them contains a N-H or a O-H functional group.
What is dipole-dipole interaction?
200
The type of DNA that is left handed.
What is Z-DNA?
200
Is a electrophoresis method of confirming the identity of RNA molecules using RNA probes specific to the target sequence.
What is a Northern blot?
200
The number of water molecules produced when 12 amino acids bond together to form a 12 amino acid peptide.
What is 11 water molecules?
200
A method of determining protein identity, and possibly concentration, that uses multiple antibodies and immobilization methods.
What is ELISA?
300
A non-covalent interaction between two nonpolar molecules, not in a water solution.
What is van der Waals or London dispersion force?
300
A protein that is used in eukaryotes to pack DNA tightly, which is involved in over-expression and silencing of genes.
What is a histone?
300
Is the type of PCR that requires a TTTTTTTT primer during the first phase of the reaction.
What is RT-PCR, or reverse transcriptase PCR?
300
The "type" of amino acid that valine, alanine, and phenylalanine are.
What is nonpolar amino acids?
300
A method in which to analyze the proteome of an individual; comparing the pattern of these between diseased and healthy patients can help identify biomarkers.
What is a 2-D gel, or a Protein Chip?
400
The pH of a solution containing 2.3 x 10^-6 M of OH-.
What is 8.36?
400
Is a type of RNA that often uses modified bases and brings amino acids to the ribosome during protein synthesis.
What is tRNA?
400
In q-PCR, as the concentration of the initial DNA sample increases the Ct value ____________ (increases, decreases, or stays the same)?
What is decreases?
400
The charge on the following peptide at physiological pH: AVCYFDVDFEAKM
What is -2?
400
Is a X-ray crystal resolution that is extremely good and unlikely to have any errors.
What is 0.5-1.5 Angstroms?
500
The pKa of a weak acid, in which the concentration of HA and A- in solution at equilibrium are .075M and .025 M at pH of 6.0.
What is 6.48?
500
A method of DNA repair that will fix thymine dimer formation that can occur after DNA is exposed to UV light.
What is nucleotide excision repair?
500
The part of the CRISPR-Cas9 experimental set-up/reagents that must be complementary to the section of DNA you want to either remove or replace.
What is the guide RNA?
500
The charge of a single tyrosine amino acid at a pH =11.
What is -2?
500
The best chromatography method one should use to purify this protein from a mixture: MCFTYASLTFYSPGHHHHHH
What is immobilized metal affinity chromatography, or Ni2+ affinity chromatography?
M
e
n
u