Balancers and Markers
This mutation causes a curled wing phenotype in adult flies.
What is CyO?
This is a DNA/RNA binding protein that mislocalizes into the cytoplasm in disease, leading to a nuclear loss of function and a cytoplasmic gain of function.
What is TDP-43?
This system, originally derived from yeast, allows for temporal and spatial control over the expression of genes of interest to generate neurodegenerative models.
What is the GAL4-UAS system?
This structure in larvae is analogous to the human spinal cord.
What is a ventral nerve cord?
This is the generation time for healthy flies in 25 degrees.
What is 10 days?
This is a genetic construct that allows lethal or sterile mutations to be stably maintained in in heterozygotes.
What is a balancer?
What is C9orf72?
The Drosophila melanogaster genome is organized into this many pairs of chromosomes.
What is 4?
The fly central nervous system develops through these three successive larval stages before pupation.
What are the first, second, and third instar larval stages?
To ensure the progeny of a cross has the desired genetic composition, these types of flies need to be collected and used in the initial cross.
What are virgin females?
This x-chromosomal mutation causes depigmentation of the eye and is used to detect the presence of genes in interest in the stock and whether a contamination occurred.
What is w1118?
These types of motor neurons are impacted in ALS.
What are upper and lower motor neurons?
This drug activates the inducible expression of the GeneSwitch genetic tool.
What is RU486?
This is a type of synapse between muscles and nerves that is impacted in ALS.
What is a neuromuscular junction?
This is a simple tool to diagram out the possible genetic composition of each progeny from a cross.
What is a Punnet square?
This is an allele that allows you to track a gene of interest or a balancer, but does not prevent recombination.
What is a dominant marker?
This is a post-translationally modified protein that is found in aggregates in post-mortem patient tissue staining.
What is phospho-TDP43?
This classic method of creating transgenic flies relies on a mobile genetic element and results in random genetic integration.
What is p-element random insertion?
This structure in adult flies is analogous to the human spinal cord.
What is a thoracic ganglion?
This type of genetics is characterized by a predictable inheritance pattern for certain traits/organisms, ie genes segregate and assort independently.
What is Mendelian Genetics?
This is an arrangement of multiple chromosomal inversions that prevents recombination and often has an obvious phenotype associated with it.
What is a balancer?
This is an antisense oligonucleotide drug that was approved by the FDA for treating SOD1-ALS in 2023.
What is tofersen (or Qalsody)?
Approximately this percentage of cells in the Drosophila central nervous system are glia.
What is 5-10%?
These are the predominant type of motor neurons in Drosophila, named for the excitatory neurotransmitter they release.
What are glutamatergic neurons?
The country from where Dr. Zarnescu hails.
Where is Romania?