Spectroscopy
Type based on absorbance of radiation from wavelengths between 190 and 780 nm
What is UV/Vis (Ultraviolet/Visible Spectroscopy)?
Separation of volatiles is based on boiling point, molecular size, and polarity
What is GC (Gas Chromatography)?
Technique uses an enzyme covalently attached to an antibody to amplify and detect molecules (antigens) of interest
What is ELISA (Enzyme-linked Immunosorbent Assay)?
The ratio of solid to total fat, related to mouthfeel and textural properties, measured using NMR
What is SFC (Solid Fat Content)?
A protein separation technique based on proteins precipitating as ionic strength is increased
What is salting out?
Type based on vibration of functional groups absorbing energy with wavelengths longer than visible light but shorter than microwaves
What is IR (Infrared Spectroscopy)?
Separation of compounds based on various physicochemical interactions including adsorption, partitioning, ion exchange, affinity, and size exclusion
What is LC (Liquid Chromatography)?
An easily used field test that has a capture antibody immobilized in a zone on a porous membrane
What is LFS (Lateral Flow Strips)?
A amount of fatty acids liberated upon hydrolysis of triglycerides, measured by titrating a sample with a base
What is FFAs (Free Fatty Acids)?
A protein separation technique where a semipermeable membrane permits passage of small molecules but not large ones (without applying pressure)
What is dialysis?
Based on molecules being fragmented and ionized, allowing for identification of compounds
What is MS (Mass Spectrometry)?
Type where stationary phase is non-polar and mobile phase is polar
What is Reversed-phase chromatography?
Most frequently used thermal analysis technique, it measures heat flux and provides structural information
What is DSC (Differential Scanning Calorimetry)?
A measure of the degree of fat unsaturation, the sample is titrated and uses a color change involving starch as the endpoint
What is IV (Iodine Value)?
What is electrophoresis? OR What is polyacrylamide gel electrophoresis (PAGE)?
Type based on atomized elements that emit energy when exposed to a plasma
What is ICP-OES (Inductively coupled plasma - optical emission spectroscopy)?
Type of quantitative analysis based on chromatographing increasing concentrations of analyte of interest and plotting peak area vs. concentration
What is external standard?
Technique that measures weight change as sample is heated, providing material composition and thermal or oxidative stability
What is TGA (Thermogravimetric Analysis)?
A measure of oxidative stability, this test heats a sample to a high temperature and bubbles air through it, then measures the conductance of trapped volatiles
What is OSI (Oil Stability Index)?
What is Protein Digestibility-Corrected Amino Acid Score (PDCAAS)?
Type based on energy absorbed and emitted by nuclei in the radio frequency range of wavelengths
What is NMR (Nuclear Magnetic Resonance)?
Type of detector able to scan a range of wavelengths every few milliseconds and generate a 3-D plot of wavelength, time, and absorbance
What is PDA (Photo Diode Array)?
Assay is the combination of polyacrylamide gel electrophoresis with an immunoassay to identify proteins
What is Western Blot?
Headspace volatiles are concentrated using SPME then separated and identified using GC-MS, this measure of lipid oxidation correlates well with sensory analysis
What is VOCs (Volatile Organic Compounds)?
A spectrophotometric determination of loss of an essential amino acid; used to evaluate effect of food processing on protein nutritional quality
What is Lysine Availability?