DNA Basics
DNA Analysis
DNA Replication
CODIS
Miscellaneous
100
What does DNA stand for?
deoxyribonucleic acid
100
What does STR stand for?
Short Tandem Repeats
100
What does PCR stand for?
Polymerase Chain Reaction
100
What does CODIS stand for?
Combined DNA Index System
100
What is a buccal swab?
Swab of the inside of the cheek
200
What are the parts of a nucleotide?
Sugar, phosphate, and N-containing base
200
What does RFLPs stand for?
Restriction Fragment Length Polymorphism
200
What is the protein that unwinds the DNA helix?
DNA Helicase
200
What is a loci?
A specific location for STR analysis
200
What part of the DNA is used in forensics?
Tandem repeats or "Junk" DNA
300
What does DNA provide instructions for?
Proteins
300
How many bases are in RFLPs?
15-35 bases, repeated 100 to 1,000 times
300
What protein builds the DNA strand in replication?
DNA Polymerase
300
How many loci are used by CODIS?
13
300
How many DNA bearing cells are required for PCR?
18
400
List the four bases for DNA and the additional base for RNA
Guanine, cytosine, adenine, thymine, and uracil
400
What methods separates strands of DNA by size?
Electrophoresis
400
What are the three steps of PCR?
Denaturing, Annealing and Extending
400
List three ways to avoid contamination.
Change gloves, substrate control, disposable forceps, own well ventilated container
400
What is Ms. V's favorite thing about DNA? a. it is a fundamental unit of heredity b. the way that it replicates c. everyone has it d. it is double helix
B! PCR is a great technology based on replication!!!!!
500
What charge does DNA have?
Negative charge
500
What is a restriction enzyme?
An enzyme that will cut DNA in specific locations. "Specialized scissors"
500
What form of DNA analysis is used with PCR?
STR
500
If someone's DNA profile matched the suspect's profile, we say that they ____ __ ___ _______.
fail to be exluded
500
Why does mitochondrial DNA last longer than regular DNA in the nucleus?
There is much more of it throughout the cell!
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