pGlo
Recombinant DNA
Protein Columns
GMOs
What's the real song?
100
What actually glowed in the pGlo lab?
the GFP Protein (not the gene, DNA does not glow)
100
What type of organism is E. coli?
Bacteria
100
During protein column chromatography: What size protein elutes out of the column FIRST
Large proteins fall past the whiffle balls first
100
What does GMO stand for?
Genetically Modified Organism
100
"I can buy myself proteins"
Flowers by Miley Cyrus
200
What is the molecule that binds to the gene and blocks/stops RNA Polymerase from transcribing a gene?
Repressor
200
What is the name of the circular piece of DNA inserted into the bacteria?
Plasmid
200
What is the purpose of doing Protein Column Chromatography?
To separate (purify) the one protein we want from everything else
200
Give an example of a GMO
Golden rice, Flavor-Saver Tomato, Bt Corn, etc.
200
"Anti-Codon"
Anti-Hero by Taylor Swift
300
What was the Inducer in our pGlo lab?
the Sugar that was mixed into the agar
300
What does GFP stand for?
Green Fluorescent Protein
300
Which protein is the smallest?
B
300
Give an example of a Pro of GMOs
Cost less, reduce use of pesticides, added nutrition
300
"Purple RNA"
"Purple Rain" by Prince
400
What is the purpose of the protein column chromatography lab after we cultured pGlo?
in order to purify/separate out only the GFP protein from everything else inside the bacteria
400
What are the ends of DNA called left by a restriction enzyme that easily bond together with complimentary base pairs?
Sticky ends
400
Which protein is their a higher concentration of?
B
400
Give an example of a Con of GMOs
possible allergic reactions, could impact the ecosystem, could lead to antibiotic resistance
400
"Master of Proteins"
"Master of Puppets" by Metallica
500
Which model represents the non-sugar plate that was not expressing the protein?
Model A
500
What is the type of molecule that cuts DNA according to a specific seqence of DNA?
Restriction Enzyme
500
Does tube #4 contain a pure sample of Protein B?
No, tube 4 also contains protein C, it has not been purified
500
Do you think the FDA (Food and Drug Administration) should ban all GMOs?
*educated answer with one supported reason provided*
500
DNA.
DNA. by Kendrick Lamar