pGlo
Temperature and Gene Expression
Protein Columns
GMOs
Vocabulary
100
What actually glowed in the pGlo lab?
the GFP Protein (not the gene, DNA does not glow)
100
This bacteria made no pigment
white
100
During protein column chromatography: What size protein elutes out of the column FIRST
Large proteins fall past the whiffle balls first
100
What does GMO stand for?
Genetically Modified Organism
100
DNA is always in the
nucleus
200
What is the molecule that binds to the gene and blocks/stops RNA Polymerase from transcribing a gene?
Repressor
200
This bacteria made some pigment
pink
200
What is the purpose of doing Protein Column Chromatography?
To separate (purify) the one protein we want from everything else
200
Give an example of a GMO
Golden rice, Flavor-Saver Tomato, Bt Corn, etc.
200
DNA codes for mRNA in this process
transcription
300
What was the Inducer in our pGlo lab?
the Sugar that was mixed into the agar
300
This bacteria made a lot of pigment
red
300
Which protein is the smallest?
B
300
Give an example of a Pro of GMOs
Cost less, reduce use of pesticides, added nutrition
300
mRNA codes for amino acids in this process
translation
400
What is the purpose of the protein column chromatography lab after we cultured pGlo?
in order to purify/separate out only the GFP protein from everything else inside the bacteria
400
True or False: the pigment made is a protein
True
400
Which protein is their a higher concentration of?
B
400
Give an example of a Con of GMOs
possible allergic reactions, could impact the ecosystem, could lead to antibiotic resistance
400
This is made from a series of amino acids
protein
500
Which model represents the non-sugar plate that was not expressing the protein?
Model A
500
This was the reason why the pigment gene was expressed sometimes
temperature
500
Does tube #4 contain a pure sample of Protein B?
No, tube 4 also contains protein C, it has not been purified
500
Do you think the FDA (Food and Drug Administration) should ban all GMOs?
*educated answer with one supported reason provided*
500
This shows that a gene was expressed
protein