PCR
Which stage of PCR happens at 55 degrees?
Annealing
What type of gel is used in gel electrophoresis?
Agarose Gel
Why do we use a microarray?
Compare DNA of two different species
What do we normally use as a vector for gene transfer?
Plasmids
What's the hardest part of a human?
Tooth (enamel)
Which stage of PCR happens at 72 degrees?
Elongation
Where do we load the DNA into?
The wells of the gel
Fluorescent markers
What do we call the new DNA formed after desired genes have been put into it?
Recombinant DNA
How many Dragon Balls are there?
Seven
What is added to the single strands during annealing?
DNA Primers
Name two things that affect how far a fragment of DNA will move along the gel
length and mass
What colour do we use to label the DNA of each species?
Red and Green
Which enzyme is used to cut the DNA?
Restriction endonucleases
What food item was once used as currency?
a. Cheese
b. Potatoes
c. Bananas
d. Peanut Butter
Cheese
Which enzyme is added to the PCR at 72 degrees?
taq polymerase
Which end of the gel does the DNA move towards?
Positive anode
What is on the plate (contains known DNA sequences)?
Probes
Which enzyme is used to seal the new gene into the vector
DNA ligase
According to Guinness World Records, what's the best-selling book of all time?
The Bible
Why do we use the taq polymerase enzyme?
Heat stable, won't denature
Name two uses of gel electrophoresis
Who did it (criminal case)? Who is the father?
If a is expressed in both species, what colour will be shown on the array?
Yellow
Which enzyme turns mRNA back to DNA?
What do you call a dinosaur with an extensive vocabulary?
A thesaurus