This is how we run things!
This specific electrophoresis technique is used to separate Short Tandem Repeats based on the number of repeats.
(capillary electrophoresis)
Cleavage by S. pyrogenes Cas9 occurs how many Base Pairs 5’ to the PAM
3
This process was discovered by Fredrick Griffith
Transformation
This enzyme joins two pieces of DNA if they are sticky ends
Ligase
How many H-bonds between Guanine and Cytosine?
3
This protein cuts double stranded DNA at or near a constant palindromic target sequence
(Restriction enzyme)
Cas9 is able to create this kind of damage to DNA
(DSB or double strand break)
They flipped a coin to determine authorship in their seminal DNA publication in Nature
(Watson & Crick)
During fertilization, this protein that normally wraps and protects DNA in sperm mitochondria is blocked from entering the mito.
TFAM
Which three nucleotides are pyrimidines?
Cytosine, Thymine, and Uracil
This helps increase the contrast between DNA and the medium it moves through by binding to it
(Dye or Stain)
Cas9 hones in on target DNAs because of this 20 nucleotide molecule
(guide RNA - best answer)
This duo used differential radioisotope labeling to determine the heritable material
(Hershey & Chase)
Bacteria will do what to prevent their own DNA being cut by REs.
(Methylate it)
How many basepairs are in 1 turn of B-form DNA?
10
The metal chelator found in most DNA running buffers is called what?
EDTA
This type of repair mechanism is used to inactivate or knock-out a gene
(Non-homologous end joining/NHEJ)
“Nuclein” was discovered by this person
(Frederick Miescher)
When bacteria replicate their DNA, how do they distinguish parent DNA from daughter DNA for repair if there are DNA mismatches?
(parent DNA is methylated)
This is the resonance configuration of Thymine in B-form DNA of 20 angstroms diameter.
Keto configuration)
This requires a 2 part answer:
The Tris buffer in DNA running gels ensures that the pH of solution is (higher or lower) than the pKa of what functional group?
Higher and Phosphate Group
This is the name of the process of how CRISPR gene editing tools are inserted into human cells (transfection or transduction)
Cannot accept transformation
The determination of the ratios of nucleotides (A, T, C, and G) in living organisms was determined using what technique?
(paper chromatography)
This functional group is characterized by a Carbon double bonded to an oxygen, with 2 R-or variable groups bonding to the Carbon
Carbonyl
If you remove the phosphate group from a nucleotide it is now called a what?
(nucleoside)