this enzyme travels in front of DNA helicase and alleviates positive supercoiling
What is DNA gyrase/topoisomerase II?
100
Draw the structure ribulose (OH group please!)
What is RNA?
100
Function of helicase?
What is "separating the double stranded DNA?"
100
Which model for the make-up of DNA ended up being adopted?
What is semi-conservative?
200
the three steps of transcription are...
What is "initiation, elongation, and termination?"
200
The idea that the amount of A equals the amount of T and the amount of G equals the amount of C is known as...
What are Chargaff's rules?
200
These bases have a two ring structure
What are purines?
200
This multiprotein complex promotes binding between sister chromatids during the S phase..
What is cohesin?
200
In Hershey and Chases's experiment, where was most of the radioactively labeled (35)S found after cell lysis?
What is the supernatant? (now tell me why =)
300
These specialized regions on a chromosome prevent chromosome shortening and help prevent translocations
What is a telomere?
300
In a denaturation and renaturation experiment, which type of sequence is most likely to renature the fastest. Why?
What are "tandem repeats?" (highly repetitive sequences)
300
In a DNA strand with the following sequence,
5'-GATACCGTCCATCAACGTGG-3' which direction is the polymerase synthesizing? (from left to right or right to left?)
What is right to left?
300
This protein synthesizes short RNA primers during DNA replication....
What is primase?
300
In your past knowledge of Genetics from previous chapters, what is an example of facultative heterochromatin?
What is a Barr Body?
400
The core exzyme is composed of 5 subunits, a2, B, B', and omega. The sixth subunit is......
Why is it important?
What is sigma factor?
400
If a segment of DNA is subjected to a 360 degree left-handed turn, the result would be?
What is negative supercoiling?
400
Tightly compacted regions of chromosomes that are not typically transcriptionally active are called...
What is heterochromatin?
400
This protein binds to the TFIID and then enables RNA polymerase to bind to the core promoter...
What is TFIIB?
400
If the parent strand is: 5'-GATACCGTCCATCAACGTGG-3' what does the newly synthesized strand look like?
What is 3'-CTATGGCAGGTAGTTGCACC-5'?
500
there are two methods of termination....what are they and give a brief description.
What is "rho dependent and rho independent?" *describe what I don't feel like typing...
500
In Markus Noll's experiment with DNAse I, he hypothesized DNAse was more likely to make cuts at linker regions. Why? Describe what his experiment taught us.
What is "the beads on a string model?" (and more details I don't want to type
500
Draw the phosphodiester bonds connecting the phosphate backbone (draw the sugar, but you can just write "base" for the nucleotide)
What is acing Cornett's exam? just kidding...3' and 5'
500
What is the function of Tus?
What is "bind to the ter sequence and prevents the advancement of the replication fork" (or some answer similar to that...)
500
What is the consensus sequence in these DNA sequences:
GGCATTGACT
GCCATTGTCA
CGCATAGTCA
GGAAATGGGA
GGCTTTGTCA
GGCATAGTCA