Pep Squad
2 amino acids can be joined in a ___________ bond
peptide
Three nucleotides of an mRNA that encode for a specific amino acid
codon
These macromolecules carry amino acids to a nascent peptide and deliver them according to their specific binding with codons
tRNA
If you PCR a gene using the DNA of a single cell, by the 6th round of PCR, you will have this many copies of the gene
128
2 copies to start
4(1 round) 8(2 rounds), 16 (3 rounds) 32 (4 rounds), 64 (5 rounds), 128 (6 rounds)
If you want to know in what cell types a gene is transcribed, you would use
in situ hybridization
The first amino acid in a peptide sequence makes up the ____________ terminus
amino/n
Different codons can encode the same amino acid, a property known as this
redundancy
Translation Initiation requires initiation factors, a Met-tRNA and this
Small subunit of the ribosome
An enzyme that converts RNA into cDNA
reverse transcriptase
This can be used to reduce the amount of transcript in cells and tissues without affecting the DNA
RNAi
The last amino acid to be added to the nascent peptide chain by the ribosome makes up the ________ terminus of the protein
carboxy/C
The first nucleotides in an mRNA are part of this structure
5'UTR
The tRNA located in this ribosomal site is attached to the nascent peptide
P site
DNA or RNA that has been converted to cDNA can be quantified using this technique
qPCR
To view levels of your favorite protein on a Western Blot, you must first develop one of these
Antibody to YFP
A beta sheet is an example of this level of protein structure
secondary
The initiation codon in eukaryotes is often situated in this type of sequence
Kozak
The energy stored in this molecule drives most of the steps of translation
GTP
DNA is isolated from a crime scene. You have 4 suspects in the crime. How would you use DNA to determine whether any of the suspects was present at the crime scene
STR analysis. PCR of known variable STRS, followed by electrophoresis and analysis
This type of repair can be used with CRISPR-Cas9 to insert a tag into your gene of interest
Homology-Driven repair
The idea of 1 gene; 1 enzyme comes from the study of these neurospora mutants that can only grow if their enzymatic product is added to the media
auxotrophs
The start codon is often this sequence on an mRNA
AUG
_______ is a protein that is shaped like a tRNA that binds stop codons and drives termination of translation
release factor
The EcoRI enzyme cuts a plasmid at a single site. How many bands would be seen on a gel from digest of this plasmid with EcoRI?
1
This method allows you to study a protein's binding partners in an intact cell
proximity labeling